BRENDA - Enzyme Database

Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase

Barta, M.L.; Lovell, S.; Sinclair, A.N.; Battaile, K.P.; Hefty, P.S.; Biochemistry 53, 214-224 (2014)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
3.6.1.17
structures of both apo- and ligand-bound CT771, to 2.6 A and 1.9 A resolution, respectively. The structure shows a alphabetaalpha-sandwich motif with many conserved elements lining the putative Nudix active site
Chlamydia trachomatis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.005
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.0073
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.0093
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.326
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.333
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.407
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+
Chlamydia trachomatis
3.6.1.17
Mn2+
in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+
Chlamydia trachomatis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Chlamydia trachomatis
-
serovar 434/Bu
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
733346
Chlamydia trachomatis
ATP + AMP
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.68
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.51
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.6
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.78
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
2.24
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
3.25
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.6.1.17
structures of both apo- and ligand-bound CT771, to 2.6 A and 1.9 A resolution, respectively. The structure shows a alphabetaalpha-sandwich motif with many conserved elements lining the putative Nudix active site
Chlamydia trachomatis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.005
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.0073
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.0093
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.326
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.333
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
0.407
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+
Chlamydia trachomatis
3.6.1.17
Mn2+
in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+
Chlamydia trachomatis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
733346
Chlamydia trachomatis
ATP + AMP
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.68
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.51
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.6
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
1.78
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
2.24
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
3.25
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.17
4.4
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
6.9
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
9.8
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
140
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
160
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
220
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.17
4.4
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
6.9
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
9.8
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MgCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
140
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 5.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
160
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 3.0 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis
3.6.1.17
220
-
diadenosine 5',5''-P1,P4-tetraphosphate
presence of 1.5 mM MnCl2, pH 7.5, 22°C
Chlamydia trachomatis