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Literature summary extracted from

  • Hai, Y.; Dugery, R.J.; Healy, D.; Christianson, D.W.
    Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme (2013), Biochemistry, 52, 9294-9309.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.8 expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.8 hanging drop vapor diffusion method, using 25% PEG (w/v) 3350, 0.1 M sodium acetate (pH 4.6) Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.8 N114H the mutant exhibits 35% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
3.5.3.8 R144A the mutant exhibits 99.3% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
3.5.3.8 R144E the mutant exhibits 99.9% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
3.5.3.8 R144K the mutant exhibits 77% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.8 9
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 20
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 40
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 90
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 160
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.8 Mn2+ contains two Mn2+ Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.8 N-formimidoyl-L-glutamate + H2O Trypanosoma cruzi
-
 L-glutamate + formamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.8 Trypanosoma cruzi Q4DSA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.8 Talon column chromatography and Q-HP anion exchange column chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.8 additional information no activity with L-arginine, agmatine, L-acetylarginine, alpha-guanidinoglutaric acid, L-homoarginine, L-2-amino-3-guanidinopropionic acid, 3-guanidinopropionic acid, and 4-guanidinobutyric acid Trypanosoma cruzi ?
-
 ?
3.5.3.8 N-formimidoyl-L-glutamate + H2O
-
 Trypanosoma cruzi L-glutamate + formamide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.8 trimer
-
 Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.5.3.8 FIGase
-
 Trypanosoma cruzi
3.5.3.8 formiminoglutamase
-
 Trypanosoma cruzi
3.5.3.8 N-formimino-L-glutamate formiminohydrolase
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.8 0.05
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 3.2
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 80
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 190
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 200
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.8 0.0052
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 0.037
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 1.2
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 3.4
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
3.5.3.8 5.2
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi