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Literature summary extracted from

  • Wei, D.; Huang, X.; Liu, J.; Tang, M.; Zhan, C.G.
    Reaction pathway and free energy profile for papain-catalyzed hydrolysis of N-acetyl-Phe-Gly 4-nitroanilide (2013), Biochemistry, 52, 5145-5154.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 Ac-L-Phe-Gly 4-nitroanilide + H2O whole hydrolysis process includes two stages: acylation and deacylation. The first step is a proton transfer to form a zwitterionic form (i.e. Cys-S-/His-H+ion-pair), and the second step is the nucleophilic attack on the carboxyl carbon of the substrate accompanied with the dissociation of 4-nitroaniline. The deacylation stage includes the nucleophilic attack of a water molecule on the carboxyl carbon of the substrate and dissociation between the carboxyl carbon of the substrate and the sulfhydryl sulfur of Cys25 side chain. The acylation is rate-limiting Carica papaya Ac-L-Phe-Gly + 4-nitroaniline
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Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain
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 Carica papaya