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Literature summary extracted from
- Identification of function and mechanistic insights of guanine deaminase from Nitrosomonas europaea: role of the C-terminal loop in catalysis (2013), Biochemistry, 52, 3512-3522.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.4.3 | in complex with 8-azaguanine, hanging drop vapor diffusion method | Nitrosomonas europaea |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.4.3 | E143X | the mutation results in a complete loss of activity | Nitrosomonas europaea |
| 3.5.4.3 | E79X | the mutation results in a complete loss of activity | Nitrosomonas europaea |
| 3.5.4.3 | additional information | the mutant form of the enzyme with the deletion of the extreme nine amino acids does not exhibit any enzyme activity | Nitrosomonas europaea |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.3 | Zn2+ | dependent on | Nitrosomonas europaea |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.3 | ammeline + H2O | Nitrosomonas europaea | - |
? | - |
? | |
| 3.5.4.3 | guanine + H2O | Nitrosomonas europaea | - |
xanthine + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.3 | Nitrosomonas europaea | Q82Y41 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.3 | ammeline + H2O | - |
Nitrosomonas europaea | ? | - |
? | |
| 3.5.4.3 | guanine + H2O | - |
Nitrosomonas europaea | xanthine + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.3 | homodimer | x-ray crystallography | Nitrosomonas europaea |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.4.3 | NE0047 | - |
Nitrosomonas europaea |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.3 | 120 | - |
guanine | pH and temperature not specified in the publication | Nitrosomonas europaea | |
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Release 2023.1 (January 2023)
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