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Literature summary extracted from

  • Glavas, N.A.; Hou, C.; Bragg, P.D.
    Involvement of histidine-91 of the beta subunit in proton translocation by the pyridine nucleotide transhydrogenase of Escherichia coli (1995), Biochemistry, 34, 7694-7702.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1 expressed in Escherichia coli JM 109 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.1 H91C the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 12% of the hydride transfer activity while proton translocation is reduced to 7% compared to the wild type enzyme Escherichia coli
7.1.1.1 H91D the mutant of the beta subunit retains 15% of the hydride transfer activity while proton translocation is reduced to 9% compared to the wild type enzyme Escherichia coli
7.1.1.1 H91K the mutant of the beta subunit is present in the NADP(H)-induced conformation even in the absence of these substrates. The mutant retains 4% of the hydride transfer activity while proton translocation is reduced to 20% compared to the wild type enzyme Escherichia coli
7.1.1.1 H91N the mutant of the beta subunit retains 80% of the hydride transfer activity while proton translocation is reduced to 7% compared to the wild type enzyme Escherichia coli
7.1.1.1 H91S the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 19% of the hydride transfer activity while proton translocation is reduced to 11% compared to the wild type enzyme Escherichia coli
7.1.1.1 H91T the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 11% of the hydride transfer activity while proton translocation is reduced to 8% compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.1 N,N'-dicyclohexylcarbodiimide about 12% residual activity after 180 min at 0.5 mM Escherichia coli
7.1.1.1 N-cyclohexyl-N'-(4-dimethylaminonaphthyl)carbodiimide about 25% residual activity after 200 min at 0.5 mM Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.1 membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1] Escherichia coli
-
NADP+ + NADH + H+[side 2]
-
r

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1]
-
Escherichia coli NADP+ + NADH + H+[side 2]
-
r

Synonyms

EC Number Synonyms Comment Organism
7.1.1.1 pyridine nucleotide transhydrogenase
-
Escherichia coli