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Literature summary extracted from

  • Requena, C.E.; Perez-Moreno, G.; Ruiz-Perez, L.M.; Vidal, A.E.; Gonzalez-Pacanowska, D.
    The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells (2014), Biochem. J., 459, 171-180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.12
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.12 E63Q catalytically inactive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.12 0.0092
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0168
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0333
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0365
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0476
-
 dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0629
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.065
-
 dTTP pH 8.0, 25°C Homo sapiens
3.6.1.12 0.0793
-
 dATP pH 8.0, 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.12 20800
-
 x * 20800, recombinant protein with His-tag, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.12 Homo sapiens Q9H773
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.12
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.12 HeLa cell
-
 Homo sapiens
-
3.6.1.12 MRC-5 cell
-
 Homo sapiens
-
3.6.1.12 TK-6 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.12 5-bromo-dCTP + H2O
-
 Homo sapiens 5-bromo-dCMP + diphosphate
-
 ?
3.6.1.12 5-formyl-dCTP + H2O
-
 Homo sapiens 5-formyl-dCMP + diphosphate
-
 ?
3.6.1.12 5-hydroxymethyl-dCTP + H2O
-
 Homo sapiens 5-hydroxymethyl-dCMP + diphosphate
-
 ?
3.6.1.12 5-iodo-dCTP + H2O
-
 Homo sapiens 5-iodo-dCMP + diphosphate
-
 ?
3.6.1.12 5-methyl-dCTP + H2O
-
 Homo sapiens 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.12 dATP + H2O
-
 Homo sapiens dAMP + diphosphate
-
 ?
3.6.1.12 dCTP + H2O
-
 Homo sapiens dCMP + diphosphate
-
 ?
3.6.1.12 dTTP + H2O
-
 Homo sapiens dTMP + diphosphate
-
 ?
3.6.1.12 additional information no substrtaes: ATP, CTP, UTP, dCDP, 5-methyl-dCDP, dGTP. Poor substrate: dUTP Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.12 ? x * 20800, recombinant protein with His-tag, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.12 XTP3-TPA
-
 Homo sapiens
3.6.1.12 XTP3-transactivated protein A
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.12 1.8
-
 dTTP pH 8.0, 25°C Homo sapiens
3.6.1.12 2
-
 dATP pH 8.0, 25°C Homo sapiens
3.6.1.12 3
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 4.8
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 5.6
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 5.7
-
 dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 6.2
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 9.3
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens

General Information

EC Number General Information Comment Organism
3.6.1.12 physiological function DCTPP1-deficient cells accumulate high levels of dCTP and are hypersensitive to exposure to the nucleoside analogues 5-iodo-2'-deoxycytidine and 5-methyl-2'-deoxycytidine Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.12 25
-
 dATP pH 8.0, 25°C Homo sapiens
3.6.1.12 28
-
 dTTP pH 8.0, 25°C Homo sapiens
3.6.1.12 90
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 119
-
 dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 143
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 256
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 322
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
3.6.1.12 371
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens