EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.142 | expressed in Escherichia coli | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.142 | 26-difluorocycloartenol | competitive inhibitor | Glycine max | |
2.1.1.142 | 26-fluorocycloartenol | competitive inhibitor | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.142 | 0.031 | - |
Cycloartenol | pH 7.5, 35°C | Glycine max | |
2.1.1.142 | 0.031 | - |
Cycloartenol | at 35°C in phosphate buffer at pH 8.0 | Glycine max | |
2.1.1.142 | 0.036 | - |
26-fluorocycloartenol | pH 7.5, 35°C | Glycine max | |
2.1.1.142 | 0.036 | - |
26-fluorocycloartenol | at 35°C in phosphate buffer at pH 8.0 | Glycine max | |
2.1.1.142 | 0.044 | - |
26-difluorocycloartenol | pH 7.5, 35°C | Glycine max | |
2.1.1.142 | 0.044 | - |
26-difluorocycloartenol | at 35°C in phosphate buffer at pH 8.0 | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.142 | S-adenosyl-L-methionine + cycloartenol | Glycine max | - |
S-adenosyl-L-homocysteine + 24(28)-methylenecycloartanol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.142 | Glycine max | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.142 | Q-Sepharose column chromatography | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.142 | (3beta,13alpha,17alpha)-3-fluorolanost-8,24-diene + S-adenosyl-L-methionine | - |
Glycine max | (3beta,13alpha,17alpha)-3-fluoro-24-methylidenelanost-8-ene + S-adenosyl-L-homocysteine | conversion into a single 24(28)-methylene product | ? | |
2.1.1.142 | 26-difluorocycloartenol + S-adenosyl-L-methionine | - |
Glycine max | 26-difluoro-25-hydroxy-24-methylcycloartanol + S-adenosyl-L-homocysteine | products are 26-difluorocyclolaudenol (monol), 26-difluoro-24(28)-methylenecycloartanol (monol) and 26-difluoro-25-hydroxy-24-methylcycloartanol (diol) distributed in a ratio of 6:4:90 at approximately 1% yield | ? | |
2.1.1.142 | 26-fluorocycloartenol + S-adenosyl-L-methionine | reaction affords a bound intermediate that converts in favour of the DELTA25(27)-olefin product via the cyclolaudenol cation formed initially during the C-24-methylation reaction | Glycine max | ? + S-adenosyl-L-homocysteine | hydrolysis of the product gives 26-fluoro-25-hydroxy-24-methylcycloartanol | ? | |
2.1.1.142 | 26-homocycloartenol + S-adenosyl-L-methionine | - |
Glycine max | 24-methylene-26-homocycloartenol + S-adenosyl-L-homocysteine | single product | ? | |
2.1.1.142 | cycloartenol + S-adenosyl-L-methionine | - |
Glycine max | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | single product | ? | |
2.1.1.142 | additional information | achieving the cyclolaudenyl cation intermediate by electrophilic alkylation of cycloartenol is significant to the overall reaction rate. The evolution of variant sterol C-24-methylation patterns is driven by competing reaction channels that have switched in algae from formation of primarily DELTA25(27) products that convert into ergosterol to, in land plants, formation of DELTA24(28) products that convert into sitosterol | Glycine max | ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 26-difluorocycloartenol | - |
Glycine max | ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 26-fluorocycloartenol | - |
Glycine max | ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + cycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + 24(28)-methylenecycloartanol | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + lanosterol | - |
Glycine max | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.142 | 24-SMT | - |
Glycine max |
2.1.1.142 | C-24-methyltransferase | - |
Glycine max |
2.1.1.142 | SMT1 | - |
Glycine max |
2.1.1.142 | sterol C-24-methyltransferase | - |
Glycine max |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.142 | 0.071 | - |
26-fluorocycloartenol | pH 7.5, 35°C | Glycine max | |
2.1.1.142 | 0.071 | - |
26-fluorocycloartenol | at 35°C in phosphate buffer at pH 8.0 | Glycine max | |
2.1.1.142 | 0.093 | - |
26-difluorocycloartenol | pH 7.5, 35°C | Glycine max | |
2.1.1.142 | 0.093 | - |
26-difluorocycloartenol | at 35°C in phosphate buffer at pH 8.0 | Glycine max |