EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.11 | additional information | no inhibition by 4-(2-butylbenzyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione and 4-(cyclopentylmethyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione | Homo sapiens | |
3.4.24.11 | thiorphan | - |
Homo sapiens | |
3.4.24.86 | 4-(2-butylbenzyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione | synthesis of the specific inhibitor, overview | Homo sapiens | |
3.4.24.86 | 4-(cyclopentylmethyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione | synthesis of the specific inhibitor, overview | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.11 | extracellular | - |
Homo sapiens | - |
- |
3.4.24.11 | additional information | the enzyme is released from endothelial cells via exosomes, the process is dependent on ADAM-17 protease | Homo sapiens | - |
- |
3.4.24.11 | soluble | - |
Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.86 | additional information | metalloprotease | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.11 | bradykinin + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.11 | Homo sapiens | P08473 | - |
- |
3.4.24.86 | Homo sapiens | P78536 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.11 | EA.hy926 cell | - |
Homo sapiens | - |
3.4.24.11 | endothelial cell | - |
Homo sapiens | - |
3.4.24.86 | EA.hy926 cell | - |
Homo sapiens | - |
3.4.24.86 | endothelial cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.11 | (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl) + H2O | bradykinin-based quenched fluorescent substrate assay | Homo sapiens | (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala + Phe-Lys-(2,4-dinitrophenyl) | - |
? | |
3.4.24.11 | bradykinin + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.11 | CALLA | - |
Homo sapiens |
3.4.24.11 | CD10 | - |
Homo sapiens |
3.4.24.11 | common acute lymphoblastic leukemia antigen | - |
Homo sapiens |
3.4.24.11 | endopeptidase-24.11 | - |
Homo sapiens |
3.4.24.11 | enkephalinase | - |
Homo sapiens |
3.4.24.11 | NEP | - |
Homo sapiens |
3.4.24.86 | a disintegrin and metalloprotease 17 | - |
Homo sapiens |
3.4.24.86 | ADAM-17 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.11 | 37 | - |
assay at | Homo sapiens |
3.4.24.86 | 22 | - |
assay at room temperature | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.11 | 6.3 | - |
assay at | Homo sapiens |
3.4.24.86 | 7.5 | - |
assay at | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.4.24.86 | 0.00432 | - |
pH 7.5, 22°C | Homo sapiens | 4-(cyclopentylmethyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione | |
3.4.24.86 | 0.00536 | - |
pH 7.5, 22°C | Homo sapiens | 4-(2-butylbenzyl)-5-(4-hydroxybenzyl)-1-[1-(2-[[6-(4-hydroxybenzyl)-2,3-dioxopiperazin-1-yl]methyl]pyrrolidin-1-yl)-3-(naphthalen-2-yl)propan-2-yl]piperazine-2,3-dione |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.11 | malfunction | treatment of cells with TPI2155-14 and TPI2155-17, specific inhibitors for ADAM-17 protease, EC 3.4.24.86, results in a significant decrease in neprilysin activity in media, implicating a possible role for ADAM-17 in neprilysin release | Homo sapiens |
3.4.24.11 | metabolism | the non-membrane bound form of neprilysin with catalytic activity has the potential to cleave substrates throughout the blood circulation with systemic effects. A likely role of exosomes and protease ADAM-17 in release of the enzyme, overview | Homo sapiens |
3.4.24.86 | physiological function | treatment of cells with TPI2155-14 and TPI2155-17, specific inhibitors for ADAM-17 protease, results in a significant decrease in non-membrane bound form of neprilysin, EC 3.4.24.11, activity in media, implicating a possible role for ADAM-17 in neprilysin release | Homo sapiens |