Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Singh, D.; Kumari, A.; Ramaswamy, S.; Ramanathan, G.
    Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2 (2014), Biochem. Biophys. Res. Commun., 445, 36-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.23 expression in Escherichia coli Diaphorobacter sp. DS2

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.23 additional information individual expression of each subunit and separate purification. The active recombinant enzyme can be reconstituted in vitro by mixing all three purified subunits Diaphorobacter sp. DS2

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.23 Diaphorobacter sp. DS2 M9PTH9 and M9PW10 and M9PSS0 and M9PV03 M9PTH9 i.e. ferredoxin reductase subunit MntAa, M9PW10 i.e. oxygenase alpha subunit MntAc, M9PSS0 i.e. ferredoxin subunit MntAb, M9PV03 i.e. oxygenase beta subunit MntAd
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.23 2,6-dinitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methyl-4-nitrocatechol + nitrite + NAD+ 84% of the activity with 3-nitrotoluene ?
1.14.12.23 2-chloronitrobenzene + NADH + O2
-
 Diaphorobacter sp. DS2 3-chlorocatechol + nitrite + NAD+ 80% of the activity with 3-nitrotoluene ?
1.14.12.23 2-nitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methylcatechol + nitrite + NAD+ 71% of the activity with 3-nitrotoluene. Products are 91% 3-methylcatechol, 9% 3-nitrobenzyl alcohol ?
1.14.12.23 3-nitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methylcatechol + 4-methylcatechol + nitrite + NAD+ products are 54% 3-methylcatechol, 39% 4-methylcatechol, 7% 3-nitrobenzyl alcohol ?
1.14.12.23 nitrobenzene + NADH + O2
-
 Diaphorobacter sp. DS2 catechol + nitrite + NAD+ 78% of the activity with 3-nitrotoluene ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.23 MntAa
-
 Diaphorobacter sp. DS2
1.14.12.23 MntAb
-
 Diaphorobacter sp. DS2
1.14.12.23 MntAc
-
 Diaphorobacter sp. DS2
1.14.12.23 MntAd
-
 Diaphorobacter sp. DS2