EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.88 | ligand-free enzyme, and complxed with inhibitors actinonin and caffeic acid phenethyl ester, hanging drop vapour diffusion method, condition screening, using 0.1 M HEPES, pH 7.5, 70% v/v MPD, mixing of 0.001 ml protein and reservoir solution each, and equilibration against 0.5 ml of reservoir solution, 17°C, X-ray diffraction structure determination and analysis at 1.66-1.70 A resolution | Helicobacter pylori |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.88 | (2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide | i.e. BB-3497 | Helicobacter pylori | |
3.5.1.88 | (2R)-N-[(2S)-1-{4-[(2H-1,3-benzodioxol-5-yl)methyl]piperazin-1-yl}-3,3-dimethyl-1-oxobutan-2-yl]-2-(cyclopentylmethyl)-3-[formyl(hydroxy)amino]propanamide | i.e. BB-83698 | Helicobacter pylori | |
3.5.1.88 | actinonin | 95% inhibition at 0.01 mM, competitive, locates near the active site and plugs the substrate tunnel to prevent the substrate from accessing the catalytic site | Helicobacter pylori | |
3.5.1.88 | caffeic acid phenethyl ester | CAPE, an active component of propolis, 75% inhibition at 0.01 mM, the competitive inhibitor blocks the substrate entrance, preventing substrate from approaching the active site, but CAPE does not have chelate interaction with HpPDF and does not disrupt the metal-dependent catalysis | Helicobacter pylori | |
3.5.1.88 | additional information | inhibition mechanism and kinetics, overview. No significant inhibition by caffeic acid, ferulic acid, chlorogenic acid, genistein, curcumin, naringenin, hesperitin, chrysin,apigenin, luteolin, kaempferol, myricetin, silibinin, and rutin at 0.01 mM | Helicobacter pylori | |
3.5.1.88 | N-formylhydroxylamine | i.e. LBM-415 | Helicobacter pylori | |
3.5.1.88 | N-[(2R)-2-(cyclopentylmethyl)-3-(2-{5-fluoro-6-[(9aS)-hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl]-2-methylpyrimidin-4-yl}hydrazinyl)-3-oxopropyl]-N-hydroxyformamide | i.e. GSK1322322 | Helicobacter pylori | |
3.5.1.88 | tert-butyl 1-[(2R)-2-[2-(hydroxyamino)-2-oxoethyl]hexanoyl]-L-prolinate | i.e. VRC-3375 | Helicobacter pylori | |
3.5.1.88 | VRC-4307 | - |
Helicobacter pylori |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.88 | Zn2+ | dependent on, metalloenzyme | Helicobacter pylori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.88 | Helicobacter pylori | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.88 | - |
Helicobacter pylori |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.5.1.88 | 0.00017 | - |
pH and temperature not specified in the publication | Helicobacter pylori | actinonin | |
3.5.1.88 | 0.00402 | - |
pH and temperature not specified in the publication | Helicobacter pylori | caffeic acid phenethyl ester |