Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chintalapati, S.; Prakash, J.S.; Singh, A.K.; Ohtani, S.; Suzuki, I.; Murata, N.; Shivaji, S.
    Desaturase genes in a psychrotolerant Nostoc sp. are constitutively expressed at low temperature (2007), Biochem. Biophys. Res. Commun., 362, 81-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.27 expressed in Escherichia coli DH10B cells Nostoc sp.
1.14.19.36 gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis Nostoc sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.36 membrane transmembrane domains at residues 43-65, 71-93, 200-222, and 231-253 Nostoc sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.27 33200
-
 x * 33200, calculated from amino acid sequence Nostoc sp.
1.14.19.36 41800
-
 x * 41800, about, sequence calculation Nostoc sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.27 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Nostoc sp.
-
 1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.36 a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Nostoc sp.
-
 a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.19.36 a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Nostoc sp.
-
 a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.27 Nostoc sp. Q704E9
-
-
1.14.19.36 Nostoc sp. Q704F0 gene desB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.27 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Nostoc sp. 1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.36 a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Nostoc sp. a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.19.36 a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Nostoc sp. a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.27 ? x * 33200, calculated from amino acid sequence Nostoc sp.
1.14.19.36 ? x * 41800, about, sequence calculation Nostoc sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.19.27 DesC2
-
 Nostoc sp.
1.14.19.36 DesB
-
 Nostoc sp.
1.14.19.36 Omega 3 acyl-lipid desaturase
-
 Nostoc sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.36 Ferredoxin
-
 Nostoc sp.

Expression

EC Number Organism Comment Expression
1.14.19.27 Nostoc sp. the transcript of, desC, does not show any up- or down-regulation of the expression of the desaturase gene, up to 150 min after shifting the culture from 25°C to 10°C additional information
1.14.19.36 Nostoc sp. antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids, but constitutive expression of desB gene when cultures grown at 25°C are shifted to 10°C up

General Information

EC Number General Information Comment Organism
1.14.19.36 metabolism the conversion of the C18 fatty acid to C18:3(9,12,15) is catalyzed in a step wise manner by DesC, DesA, and DesB, respectively Nostoc sp.
1.14.19.36 additional information antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids Nostoc sp.