BRENDA - Enzyme Database

Desaturase genes in a psychrotolerant Nostoc sp. are constitutively expressed at low temperature

Chintalapati, S.; Prakash, J.S.; Singh, A.K.; Ohtani, S.; Suzuki, I.; Murata, N.; Shivaji, S.; Biochem. Biophys. Res. Commun. 362, 81-87 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.27
expressed in Escherichia coli DH10B cells
Nostoc sp.
1.14.19.36
gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis
Nostoc sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.36
membrane
transmembrane domains at residues 43-65, 71-93, 200-222, and 231-253
Nostoc sp.
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.27
33200
-
x * 33200, calculated from amino acid sequence
Nostoc sp.
1.14.19.36
41800
-
x * 41800, about, sequence calculation
Nostoc sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.27
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Nostoc sp.
-
1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.36
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Nostoc sp.
-
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
1.14.19.36
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Nostoc sp.
-
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.27
Nostoc sp.
Q704E9
-
-
1.14.19.36
Nostoc sp.
Q704F0
gene desB
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.27
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733245
Nostoc sp.
1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.36
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
733245
Nostoc sp.
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.19.36
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
733245
Nostoc sp.
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.27
?
x * 33200, calculated from amino acid sequence
Nostoc sp.
1.14.19.36
?
x * 41800, about, sequence calculation
Nostoc sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.36
Ferredoxin
-
Nostoc sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.27
expressed in Escherichia coli DH10B cells
Nostoc sp.
1.14.19.36
gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis
Nostoc sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.36
Ferredoxin
-
Nostoc sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.36
membrane
transmembrane domains at residues 43-65, 71-93, 200-222, and 231-253
Nostoc sp.
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.27
33200
-
x * 33200, calculated from amino acid sequence
Nostoc sp.
1.14.19.36
41800
-
x * 41800, about, sequence calculation
Nostoc sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.27
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Nostoc sp.
-
1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.36
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Nostoc sp.
-
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
1.14.19.36
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Nostoc sp.
-
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.27
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733245
Nostoc sp.
1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.36
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
733245
Nostoc sp.
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.19.36
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
733245
Nostoc sp.
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.27
?
x * 33200, calculated from amino acid sequence
Nostoc sp.
1.14.19.36
?
x * 41800, about, sequence calculation
Nostoc sp.
Expression
EC Number
Organism
Commentary
Expression
1.14.19.27
Nostoc sp.
the transcript of, desC, does not show any up- or down-regulation of the expression of the desaturase gene, up to 150 min after shifting the culture from 25°C to 10°C
additional information
1.14.19.36
Nostoc sp.
antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids, but constitutive expression of desB gene when cultures grown at 25°C are shifted to 10°C
up
General Information
EC Number
General Information
Commentary
Organism
1.14.19.36
metabolism
the conversion of the C18 fatty acid to C18:3(9,12,15) is catalyzed in a step wise manner by DesC, DesA, and DesB, respectively
Nostoc sp.
1.14.19.36
additional information
antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids
Nostoc sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.36
metabolism
the conversion of the C18 fatty acid to C18:3(9,12,15) is catalyzed in a step wise manner by DesC, DesA, and DesB, respectively
Nostoc sp.
1.14.19.36
additional information
antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids
Nostoc sp.
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.19.27
Nostoc sp.
the transcript of, desC, does not show any up- or down-regulation of the expression of the desaturase gene, up to 150 min after shifting the culture from 25°C to 10°C
additional information
1.14.19.36
Nostoc sp.
antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids, but constitutive expression of desB gene when cultures grown at 25°C are shifted to 10°C
up