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Literature summary extracted from

  • Bajaj, B.; Sharma, N.; Singh, S.
    Enhanced production of fibrinolytic protease from Bacillus cereus NS-2 using cotton seed cake as nitrogen source (2013), Biocatal. Agricult. Biotechnol., 2, 204-209.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 Co2+ 21.6% inhibition at 10 mM Bacillus cereus
3.4.24.72 Hg2+ 63% inhibition at 10 mM Bacillus cereus
3.4.24.72 K+ 30.9% inhibition at 10 mM Bacillus cereus
3.4.24.72 Pb2+ 85.5% inhibition at 10 mM Bacillus cereus
3.4.24.72 SDS 29% residual activity at 0.01% (w/v) Bacillus cereus
3.4.24.72 Triton X-100 87% residual activity at 0.01% (v/v) Bacillus cereus
3.4.24.72 Tween 20 80% residual activity at 0.01% (v/v) Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Ca2+ 44.4% increase of activity at 10 mM Bacillus cereus
3.4.24.72 Cu2+ 34.2% increase of activity at 10 mM Bacillus cereus
3.4.24.72 Fe2+ 76.7% increase of activity at 10 mM Bacillus cereus
3.4.24.72 Mg2+ 29% increase of activity at 10 mM Bacillus cereus
3.4.24.72 Mn2+ 38% increase of activity at 10 mM Bacillus cereus
3.4.24.72 Zn2+ 37.9% increase of activity at 10 mM Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 Fibrin + H2O Bacillus cereus
-
 ?
-
 ?
3.4.24.72 Fibrin + H2O Bacillus cereus NS-2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Bacillus cereus
-
-
-
3.4.24.72 Bacillus cereus NS-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 ammonium sulfate precipitation, DEAE-Sepharose column chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 Fibrin + H2O
-
 Bacillus cereus ?
-
 ?
3.4.24.72 Fibrin + H2O
-
 Bacillus cereus NS-2 ?
-
 ?
3.4.24.72 Gelatin + H2O
-
 Bacillus cereus ?
-
 ?
3.4.24.72 Gelatin + H2O
-
 Bacillus cereus NS-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 fibrinolytic protease
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 40
-
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.72 20 80 fibrinolytic protease loses about 40-60% of its activity after 1 h incubation at 20-80°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 9
-
-
 Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.72 4 12 pre-incubation of fibrinolytic protease for 1 h at pH 4.0-12.0 causes substantial activity loss Bacillus cereus

Expression

EC Number Organism Comment Expression
3.4.24.72 Bacillus cereus cotton cake as nitrogen source enhances fibrinolytic protease production substantially (71%) as compared to control up