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Literature summary extracted from

  • El-Sayed, A.S.; Shindia, A.A.; Diab, A.A.; Rady, A.M.
    Purification and immobilization of L-arginase from thermotolerant Penicillium chrysogenum KJ185377.1; with unique kinetic properties as thermostable anticancer enzyme (2014), Arch. Pharm. Res., 0, doi.org/10.1007/s12272-014-0498-y.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.1 hydroxylamine
-
 Penicillium chrysogenum
3.5.3.1 iodoacetamide
-
 Penicillium chrysogenum
3.5.3.1 propargylglycine
-
 Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 4.8
-
 L-arginine free and chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 15.2
-
 L-arginine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 22.1
-
 L-phenylalanine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 32.8
-
 L-phenylalanine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 57.5
-
 L-phenylalanine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Mn2+ required Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 37000
-
 x * 37000, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.1 L-arginine + H2O Penicillium chrysogenum
-
 L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O Penicillium chrysogenum KJ185377.1
-
 L-ornithine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Penicillium chrysogenum
-
-
-
3.5.3.1 Penicillium chrysogenum KJ185377.1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1 ammonium sulfate precipitation, DEAE-Sepharose column chromatography and gel filtration Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.1 2.44
-
 crude enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 24.45
-
 after 10.02fold purification, at pH 7.0 and 40°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Penicillium chrysogenum L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Penicillium chrysogenum KJ185377.1 L-ornithine + urea
-
 ?
3.5.3.1 L-phenylalanine + H2O
-
 Penicillium chrysogenum 2-oxo-3-phenylpropanoate + NH3
-
 ?
3.5.3.1 L-phenylalanine + H2O
-
 Penicillium chrysogenum KJ185377.1 2-oxo-3-phenylpropanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.1 ? x * 37000, SDS-PAGE Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.5.3.1 ARG
-
 Penicillium chrysogenum
3.5.3.1 L-arginine amidinohydrolase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.1 50
-
-
 Penicillium chrysogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.1 0.18
-
 L-arginine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.25
-
 L-arginine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.25
-
 L-phenylalanine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.27
-
 L-arginine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.46
-
 L-phenylalanine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.94
-
 L-phenylalanine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.1 6.8 7.9
-
 Penicillium chrysogenum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.1 0.0305
-
 propargylglycine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0362
-
 iodoacetamide chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0442
-
 hydroxylamine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0618
-
 iodoacetamide free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0626
-
 propargylglycine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0656
-
 hydroxylamine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0658
-
 iodoacetamide PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0808
-
 hydroxylamine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
3.5.3.1 0.0857
-
 propargylglycine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.1 0.0618
-
 free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum iodoacetamide
3.5.3.1 0.0626
-
 PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum propargylglycine
3.5.3.1 0.0656
-
 PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum hydroxylamine
3.5.3.1 0.0658
-
 PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum iodoacetamide
3.5.3.1 0.0808
-
 free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum hydroxylamine
3.5.3.1 0.0857
-
 free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum propargylglycine