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Literature summary extracted from

  • Ball, J.; Bui, Q.V.; Gannavaram, S.; Gadda, G.
    Importance of glutamate 87 and the substrate alpha-amine for the reaction catalyzed by D-arginine dehydrogenase (2015), Arch. Biochem. Biophys., 568, 56-63.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.99.6 E87L kcat/Km pH-profile of the E87L mutant indicates only a single unprotonated group is required for maximal activity with D-arginine. E87 is the unprotonated group on the enzyme that binds cationic substrates Pseudomonas aeruginosa
1.4.99.6 H48F residue H48 is not responsible for the observed pKa value Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 1.8
-
 D-leucine wild-type, pH 6.0, 25°C Pseudomonas aeruginosa
1.4.99.6 4.6
-
 D-leucine wild-type, pH 10.0, 25°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Pseudomonas aeruginosa Q9HXE3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 D-arginine + phenazine methosulfate
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate overall reaction ?
1.4.99.6 D-leucine + phenazine methosulfate
-
 Pseudomonas aeruginosa ? + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-lysine + phenazine methosulfate
-
 Pseudomonas aeruginosa 6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate
-
 ?
1.4.99.6 D-methionine + phenazine methosulfate
-
 Pseudomonas aeruginosa 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.99.6 0.061
-
 D-leucine wild-type, pH 6.0, 25°C Pseudomonas aeruginosa
1.4.99.6 55
-
 D-leucine wild-type, pH 10.0, 25°C Pseudomonas aeruginosa
1.4.99.6 160
-
 D-arginine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 190
-
 D-methionine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 550
-
 D-Lysine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.99.6 0.033
-
 D-leucine wild-type, pH 6.0, 25°C Pseudomonas aeruginosa
1.4.99.6 12
-
 D-leucine wild-type, pH 10.0, 25°C Pseudomonas aeruginosa
1.4.99.6 36
-
 D-methionine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 140
-
 D-arginine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, 6°C Pseudomonas aeruginosa
1.4.99.6 150
-
 D-arginine mutant E87L, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 580
-
 D-Lysine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 1100
-
 D-arginine mutant H48F, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa
1.4.99.6 2600
-
 D-arginine wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa