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Nicotinamide N-methyltransferase enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells

Xie, X.; Yu, H.; Wang, Y.; Zhou, Y.; Li, G.; Ruan, Z.; Li, F.; Wang, X.; Liu, H.; Zhang, J.; Arch. Biochem. Biophys. 564, 52-66 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
recombinant overexpression of enzyme NNMT in colorectal cancer cells line SW480 which lacks constitutive NNMT expression, quantitative real-time PCR analysis
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.1
additional information
downregulation of enzyme NNMT expression in HT-29 cells. Balb/c mice are injected with NNMT-transfected SW-480 cells, the enzyme significantly accelerates cell proliferation and enhances tumorigenicity in mice
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.1
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
colorectal cancer cell
-
Homo sapiens
-
2.1.1.1
HT-29 cell
exhibit high endogenous expression of enzyme NNMT
Homo sapiens
-
2.1.1.1
additional information
nicotinamide N-methyltransferase is overexpressed in several malignancies, including colorectal cancer cells. No constitutive expresion of the enzyme in colorectal cancer cells line SW480
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733214
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
recombinant overexpression of enzyme NNMT in colorectal cancer cells line SW480 which lacks constitutive NNMT expression, quantitative real-time PCR analysis
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.1
additional information
downregulation of enzyme NNMT expression in HT-29 cells. Balb/c mice are injected with NNMT-transfected SW-480 cells, the enzyme significantly accelerates cell proliferation and enhances tumorigenicity in mice
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.1
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
colorectal cancer cell
-
Homo sapiens
-
2.1.1.1
HT-29 cell
exhibit high endogenous expression of enzyme NNMT
Homo sapiens
-
2.1.1.1
additional information
nicotinamide N-methyltransferase is overexpressed in several malignancies, including colorectal cancer cells. No constitutive expresion of the enzyme in colorectal cancer cells line SW480
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733214
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.1.1.1
physiological function
nicotinamide N-methyltransferase is involved in the biotransformation and detoxification of many drugs and xenobiotic compounds, it enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells. The enzyme significantly accelerates cell proliferation, enhances colony formation in vitro and tumorigenicity in mice, and inhibits apoptosis, it promotes cell cycle progression, increases ATP and 1-methylnicotinamide level and decreases ROS levels. 1-Methylnicotinamide also accelerates cell growth, inhibits apoptosis, promotes cell cycle progression, attenuates ROS production and increases ATP levels. The enzyme may play a vital role in energy balance and ROS induction, 1-Methylnicotinamide is beneficial in conditions such as inflammation and antioxidation
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.1
physiological function
nicotinamide N-methyltransferase is involved in the biotransformation and detoxification of many drugs and xenobiotic compounds, it enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells. The enzyme significantly accelerates cell proliferation, enhances colony formation in vitro and tumorigenicity in mice, and inhibits apoptosis, it promotes cell cycle progression, increases ATP and 1-methylnicotinamide level and decreases ROS levels. 1-Methylnicotinamide also accelerates cell growth, inhibits apoptosis, promotes cell cycle progression, attenuates ROS production and increases ATP levels. The enzyme may play a vital role in energy balance and ROS induction, 1-Methylnicotinamide is beneficial in conditions such as inflammation and antioxidation
Homo sapiens