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Literature summary extracted from

  • Wu, Q.; McLeish, M.J.
    Kinetic and pH studies on human phenylethanolamine N-methyltransferase (2013), Arch. Biochem. Biophys., 539, 1-8.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.28 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline i.e. SK&F 64139 Homo sapiens
2.1.1.28 7-nitro-1,2,3,4-tetrahydroisoquinoline
-
 Homo sapiens
2.1.1.28 8,9-dichloro-2,3,4,5-tetrahydro-1H-2-benzazepine i.e. LY 134046 Homo sapiens
2.1.1.28 SKF 29661
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.28 0.00054
-
 3-trifluoromethylphenylethanolamine pH 8.0, 30°C Homo sapiens
2.1.1.28 0.0012
-
 S-adenosyl-L-methionine cosubstrate 3-trifluoromethylphenylethanolamine, pH 8.0, 30°C Homo sapiens
2.1.1.28 0.0034
-
 S-adenosyl-L-methionine cosubstrate phenylethanolamine, pH 8.0, 30°C Homo sapiens
2.1.1.28 0.0055
-
 octopamine pH 8.0, 30°C Homo sapiens
2.1.1.28 0.0067
-
 S-adenosyl-L-methionine cosubstrate octopamine, pH 8.0, 30°C Homo sapiens
2.1.1.28 0.1
-
 phenylethanolamine pH 8.0, 30°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.28 Homo sapiens P11086
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.28 3-trifluoromethylphenylethanolamine + S-adenosyl-L-methionine
-
 Homo sapiens N-methyl-3-trifluoromethylphenylethanolamine + S-adenosyl-L-homocysteine
-
 ?
2.1.1.28 additional information enzyme operates by an ordered sequential mechanism in which AdoMet binds first. A protonated and an unprotonated group are required for catalysis Homo sapiens ?
-
 ?
2.1.1.28 octopamine + S-adenosyl-L-methionine
-
 Homo sapiens N-methyloctopamine + S-adenosyl-L-homocysteine
-
 ?
2.1.1.28 phenylethanolamine + S-adenosyl-L-methionine
-
 Homo sapiens N-methylphenylethanolamine + S-adenosyl-L-homocysteine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.28 0.008
-
 3-trifluoromethylphenylethanolamine pH 8.0, 30°C Homo sapiens
2.1.1.28 0.021
-
 octopamine pH 8.0, 30°C Homo sapiens
2.1.1.28 0.047
-
 phenylethanolamine pH 8.0, 30°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.28 0.0000016
-
 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline pH 8.0, 30°C Homo sapiens
2.1.1.28 0.0000044
-
 8,9-dichloro-2,3,4,5-tetrahydro-1H-2-benzazepine pH 8.0, 30°C Homo sapiens
2.1.1.28 0.000078
-
 7-nitro-1,2,3,4-tetrahydroisoquinoline pH 8.0, 30°C Homo sapiens
2.1.1.28 0.00012
-
 SKF 29661 pH 8.0, 30°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.28 0.467
-
 phenylethanolamine pH 8.0, 30°C Homo sapiens
2.1.1.28 3.67
-
 octopamine pH 8.0, 30°C Homo sapiens
2.1.1.28 15.5
-
 3-trifluoromethylphenylethanolamine pH 8.0, 30°C Homo sapiens