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Literature summary extracted from

  • Lee, H.; Park, J.; Jung, C.; Han, D.; Seo, J.; Ahn, J.H.; Chong, Y.; Hur, H.G.
    Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis (2015), Appl. Microbiol. Biotechnol., 99, 9473-9481.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.102 expression in Escherichia coli Enterobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.102 D112N 121% of wild-type activity Enterobacter sp.
4.1.1.102 F95A 120% of wild-type activity Enterobacter sp.
4.1.1.102 F95A/D112N 395% of wild-type activity Enterobacter sp.
4.1.1.102 F95A/S157I 218% of wild-type activity Enterobacter sp.
4.1.1.102 F95A/V151I 333% of wild-type activity Enterobacter sp.
4.1.1.102 F95E 26% of wild-type activity Enterobacter sp.
4.1.1.102 F95I 148% of wild-type activity Enterobacter sp.
4.1.1.102 F95I/D112N 418% of wild-type activity Enterobacter sp.
4.1.1.102 F95I/S157I 299% of wild-type activity Enterobacter sp.
4.1.1.102 F95I/V151I 323% of wild-type activity Enterobacter sp.
4.1.1.102 F95K 18% of wild-type activity Enterobacter sp.
4.1.1.102 F95L 188% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/D112N 485% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/D112N/S157I 430% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/D112N/V151I 1354% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/D112N/V151I approximately 34fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid, possibly due to formation of a compact active site compared with that of the wild-type Enterobacter sp.
4.1.1.102 F95L/S157I 528% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/V151I 746% of wild-type activity Enterobacter sp.
4.1.1.102 F95L/V151I/S157I 484% of wild-type activity Enterobacter sp.
4.1.1.102 F95Q 48% of wild-type activity Enterobacter sp.
4.1.1.102 F95R 19% of wild-type activity Enterobacter sp.
4.1.1.102 S157I 171% of wild-type activity Enterobacter sp.
4.1.1.102 V151I 174% of wild-type activity Enterobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.102 Co2+ 1 mM, 62.9% residual activity Enterobacter sp.
4.1.1.102 Cu2+ 1 mM, almost complete loss of activity Enterobacter sp.
4.1.1.102 Fe2+ 1 mM, 94.3% residual activity Enterobacter sp.
4.1.1.102 Fe3+ 1 mM, 1.7% residual activity Enterobacter sp.
4.1.1.102 Mg2+ 1 mM, 69.7% residual activity Enterobacter sp.
4.1.1.102 Mn2+ 1 mM, 67.9% residual activity Enterobacter sp.
4.1.1.102 Ni2+ 1 mM, 81.8% residual activity Enterobacter sp.
4.1.1.102 Zn2+ 1 mM, almost complete loss of activity Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.102 Enterobacter sp. C6F3U5
-
-
4.1.1.102 Enterobacter sp. Px6-4 C6F3U5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.102 16.3
-
 wild-type, pH 7.0, 37°C Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.102 ferulate
-
 Enterobacter sp. 4-vinylguaiacol + CO2
-
 ?
4.1.1.102 ferulate
-
 Enterobacter sp. Px6-4 4-vinylguaiacol + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.102 FADase
-
 Enterobacter sp.