BRENDA - Enzyme Database

Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids

Han, Y.B.; Wu, L.; Rich, J.R.; Huang, F.T.; Withers, S.G.; Feng, Y.; Yang, G.Y.; Appl. Microbiol. Biotechnol. 99, 6715-6726 (2015)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.69
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Application
EC Number
Application
Commentary
Organism
3.5.1.69
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Pseudomonas sp.
3.5.1.69
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Shewanella algae
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.5.1.69
the gene encoding the mature SA_SCD protein, which lacks its 38-residue N-terminal secretion signal sequence and from which the 277-residue C-terminal sequence is deleted, is codon-optimized and expressed in Escherichia coli strain BL21 (DE3) pLysS as Histagged enzyme
Shewanella algae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.69
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Cu2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
deoxycholic acid
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
3.5.1.69
dimethoxyethane
addition of 5% of DME strongly promoted the activity, further increasing the DME concentrations progressively inhibited synthetic activity
Shewanella algae
3.5.1.69
DMSO
low concentrations of DMSO, up to a maximum at 10%, enhance synthetic activity, while higher concentrations inhibit
Shewanella algae
3.5.1.69
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
taurodeoxycholate
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
3.5.1.69
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
3.5.1.69
Zn2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.69
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
additional information
poor effect by EDTA
Shewanella algae
3.5.1.69
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.1.69
Pseudomonas sp.
-
-
-
3.5.1.69
Shewanella algae
Q8RTY6
-
-
3.5.1.69
Shewanella algae G8
Q8RTY6
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.1.69
recombinant His-tagged enzyme, lacking the secretion signal, from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
Shewanella algae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.69
commercial preparation
-
Pseudomonas sp.
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
arachidic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
arachidic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
behenic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
behenic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
caproic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
caproic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
caprylic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
caprylic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
decanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
decanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
docosahexaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
docosahexaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
eicosapentaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
eicosapentaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
GM1a + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM1a
-
-
-
r
3.5.1.69
GM1a + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM1a
-
-
-
r
3.5.1.69
GM3 + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM3
-
-
-
r
3.5.1.69
GM3 + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM3
-
-
-
r
3.5.1.69
hexadecanedioic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
lauric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
lauric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
lignoceric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
lignoceric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. PS_SCD also prefers glycosphingolipids possessing larger sugar moieties, but it does not show preference towards glycosphingolipids with charged head groups, the enzyme hydrolyzes sulfatide faster than GM1a and GM3, specificity of the hydrolytic and synthetic reactions of PS_SCD for hydrophilic head groups, overview
733197
Pseudomonas sp.
?
-
-
-
?
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae
?
-
-
-
?
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae G8
?
-
-
-
?
3.5.1.69
myristic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
myristic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
oleic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
oleic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-azido palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-azido palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
Synonyms
EC Number
Synonyms
Commentary
Organism
3.5.1.69
PS_SCD
-
Pseudomonas sp.
3.5.1.69
SA_SCD
-
Shewanella algae
3.5.1.69
SCDase
-
Pseudomonas sp.
3.5.1.69
SCDase
-
Shewanella algae
3.5.1.69
sphingolipid ceramide N-deacylase
-
Pseudomonas sp.
3.5.1.69
sphingolipid ceramide N-deacylase
-
Shewanella algae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.69
37
-
assay at
Pseudomonas sp.
3.5.1.69
37
-
assay at
Shewanella algae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.69
6
-
assay at, forward direction
Pseudomonas sp.
3.5.1.69
6
-
hydrolytic activity optimum
Shewanella algae
3.5.1.69
7.5
-
assay at, reverse direction
Pseudomonas sp.
3.5.1.69
7.5
-
synthetic activity optimum
Shewanella algae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.69
5
9
activity range, hydrolysis
Shewanella algae
3.5.1.69
5.5
10
activity range, synthesis
Shewanella algae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.69
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.1.69
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Pseudomonas sp.
3.5.1.69
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Shewanella algae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.69
the gene encoding the mature SA_SCD protein, which lacks its 38-residue N-terminal secretion signal sequence and from which the 277-residue C-terminal sequence is deleted, is codon-optimized and expressed in Escherichia coli strain BL21 (DE3) pLysS as Histagged enzyme
Shewanella algae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.69
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Cu2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
deoxycholic acid
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
3.5.1.69
dimethoxyethane
addition of 5% of DME strongly promoted the activity, further increasing the DME concentrations progressively inhibited synthetic activity
Shewanella algae
3.5.1.69
DMSO
low concentrations of DMSO, up to a maximum at 10%, enhance synthetic activity, while higher concentrations inhibit
Shewanella algae
3.5.1.69
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
taurodeoxycholate
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
3.5.1.69
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
3.5.1.69
Zn2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.69
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
3.5.1.69
additional information
poor effect by EDTA
Shewanella algae
3.5.1.69
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.69
recombinant His-tagged enzyme, lacking the secretion signal, from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
Shewanella algae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.69
commercial preparation
-
Pseudomonas sp.
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
3.5.1.69
arachidic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
arachidic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
behenic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
behenic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
caproic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
caproic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
caprylic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
caprylic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
decanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
decanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
docosahexaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
docosahexaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
eicosapentaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
eicosapentaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
GM1a + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM1a
-
-
-
r
3.5.1.69
GM1a + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM1a
-
-
-
r
3.5.1.69
GM3 + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM3
-
-
-
r
3.5.1.69
GM3 + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM3
-
-
-
r
3.5.1.69
hexadecanedioic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
lauric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
lauric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
lignoceric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
lignoceric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. PS_SCD also prefers glycosphingolipids possessing larger sugar moieties, but it does not show preference towards glycosphingolipids with charged head groups, the enzyme hydrolyzes sulfatide faster than GM1a and GM3, specificity of the hydrolytic and synthetic reactions of PS_SCD for hydrophilic head groups, overview
733197
Pseudomonas sp.
?
-
-
-
?
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae
?
-
-
-
?
3.5.1.69
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae G8
?
-
-
-
?
3.5.1.69
myristic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
myristic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
oleic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
oleic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-azido palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-azido palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
3.5.1.69
palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
3.5.1.69
palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.69
37
-
assay at
Pseudomonas sp.
3.5.1.69
37
-
assay at
Shewanella algae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.69
6
-
assay at, forward direction
Pseudomonas sp.
3.5.1.69
6
-
hydrolytic activity optimum
Shewanella algae
3.5.1.69
7.5
-
assay at, reverse direction
Pseudomonas sp.
3.5.1.69
7.5
-
synthetic activity optimum
Shewanella algae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.69
5
9
activity range, hydrolysis
Shewanella algae
3.5.1.69
5.5
10
activity range, synthesis
Shewanella algae