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Literature summary extracted from
- Improvement of the amides forming capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 by site-directed mutagenesis (2015), Appl. Microbiol. Biotechnol., 99, 2623-2635.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.5 | expressed in Escherichia coli JM109 cells | Pseudomonas fluorescens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.5.5 | E137A | the mutant demonstrates less than 1% of the wild type activity but is still enzymatically competent to convert mandelonitrile to mandelic acid and mandeloamide | Pseudomonas fluorescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.5 | Pseudomonas fluorescens | - |
- |
- |
| 3.5.5.5 | Pseudomonas fluorescens EBC191 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens | (S)-2-phenylpropionic acid + NH3 | ee 66% | ? |  |
| 3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (S)-2-phenylpropionic acid + NH3 | ee 66% | ? | |
| 3.5.5.5 | (R,S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens | (R)-mandelic acid + NH3 | ee 22% | ? | |
| 3.5.5.5 | (R,S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (R)-mandelic acid + NH3 | ee 22% | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.5.5 | Arylacetonitrilase | - |
Pseudomonas fluorescens |
| 3.5.5.5 | NitA | - |
Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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