show Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726
Ma, Y.; Xu, W.; Zhang, J.; Zhang, S.; Hong, K.; Deng, Z.; Sun, Y.; Appl. Microbiol. Biotechnol. 98, 8583-8590 (2014)
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.4
PMSF
slight activation at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
PMSF
slight activation at 1 mM
Streptomyces sp.
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.4
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces sp.
3.5.1.13
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Streptomyces sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.4
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.4
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
2.3
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
39.4
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
45.1
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
135.6
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.4
additional information
no requirement for metal ions
Streptomyces sp.
3.5.1.13
additional information
no requirement for metal ions
Streptomyces sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
31000
-
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
31000
-
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.4
4-guanidinobutyramide + H2O
Streptomyces sp.
probably the natural substrate
?
-
-
?
3.5.1.13
additional information
Streptomyces sp.
4-guanidinobutyramide probably is the natural substrate
?
-
-
-
3.5.1.13
additional information
Streptomyces sp. 211726
4-guanidinobutyramide probably is the natural substrate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Streptomyces sp.
A0A088BHP3
gene azl13
-
3.5.1.4
Streptomyces sp. 211726
A0A088BHP3
gene azl13
-
3.5.1.13
Streptomyces sp.
A0A088BHP3
gene azl13
-
3.5.1.13
Streptomyces sp. 211726
A0A088BHP3
gene azl13
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.4
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
3.5.1.13
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.4
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
-
3.5.1.13
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.4
0.55
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.4
0.69
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.4
0.87
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 4'-methylacetanilide
Streptomyces sp.
3.5.1.13
0.55
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.13
0.69
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.13
0.87
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide
Streptomyces sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp.
2,6-dimethylaniline + acetate
-
-
-
?
3.5.1.4
3',4'-dichloropropionanilide + H2O
-
733190
Streptomyces sp.
3,4-dichloroaniline + propanoate
-
-
-
?
3.5.1.4
4'-methylacetanilide + H2O
-
733190
Streptomyces sp.
4-methylaniline + acetate
-
-
-
?
3.5.1.4
4-guanidinobutyramide + H2O
best substrate
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.4
4-guanidinobutyramide + H2O
probably the natural substrate
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.4
acetamide + H2O
-
733190
Streptomyces sp.
acetate + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
733190
Streptomyces sp.
benzoate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
733190
Streptomyces sp.
isobutyrate + NH3
-
-
-
?
3.5.1.4
isonicotinamide + H2O
-
733190
Streptomyces sp.
isonicotinate + NH3
-
-
-
?
3.5.1.4
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows aryl acylamide hydrolase activity, EC 3.5.1.13, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with L-glutamine and L-asparagine
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
reaction of EC 3.5.1.19, nicotinamidase
733190
Streptomyces sp.
nicotinate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
733190
Streptomyces sp.
propionate + NH3
-
-
-
?
3.5.1.4
pyrazinamide + H2O
-
733190
Streptomyces sp.
pyrazinate + NH3
-
-
-
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp. 211726
?
-
-
-
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp.
3,4-dichloroaniline + propionate
-
-
-
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp. 211726
3,4-dichloroaniline + propionate
-
-
-
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp. 211726
?
-
-
-
-
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp. 211726
?
-
-
-
-
3.5.1.13
p-acetotoluidide + H2O
-
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.13
p-acetotoluidide + H2O
-
733190
Streptomyces sp. 211726
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
40
-
-
Streptomyces sp.
3.5.1.13
40
-
-
Streptomyces sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
15
60
activity range
Streptomyces sp.
3.5.1.13
15
60
activity range
Streptomyces sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
40
-
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.4
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.4
60
-
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
3.5.1.13
40
-
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.13
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.13
60
-
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
5.6
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
28.6
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
29.9
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
55.6
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
8
-
-
Streptomyces sp.
3.5.1.13
8
-
-
Streptomyces sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
5.5
10
activity range
Streptomyces sp.
3.5.1.13
5.5
10
activity range
Streptomyces sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.4
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
3.5.1.13
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.4
PMSF
slight activation at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
PMSF
slight activation at 1 mM
Streptomyces sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces sp.
3.5.1.13
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Streptomyces sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.4
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.4
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
2.3
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
39.4
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
45.1
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
135.6
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.4
additional information
no requirement for metal ions
Streptomyces sp.
3.5.1.13
additional information
no requirement for metal ions
Streptomyces sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
31000
-
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
31000
-
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.4
4-guanidinobutyramide + H2O
Streptomyces sp.
probably the natural substrate
?
-
-
?
3.5.1.13
additional information
Streptomyces sp.
4-guanidinobutyramide probably is the natural substrate
?
-
-
-
3.5.1.13
additional information
Streptomyces sp. 211726
4-guanidinobutyramide probably is the natural substrate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
3.5.1.13
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.4
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
-
3.5.1.13
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.4
0.55
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.4
0.69
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.4
0.87
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 4'-methylacetanilide
Streptomyces sp.
3.5.1.13
0.55
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.13
0.69
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.13
0.87
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp.
2,6-dimethylaniline + acetate
-
-
-
?
3.5.1.4
3',4'-dichloropropionanilide + H2O
-
733190
Streptomyces sp.
3,4-dichloroaniline + propanoate
-
-
-
?
3.5.1.4
4'-methylacetanilide + H2O
-
733190
Streptomyces sp.
4-methylaniline + acetate
-
-
-
?
3.5.1.4
4-guanidinobutyramide + H2O
best substrate
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.4
4-guanidinobutyramide + H2O
probably the natural substrate
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.4
acetamide + H2O
-
733190
Streptomyces sp.
acetate + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
733190
Streptomyces sp.
benzoate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
733190
Streptomyces sp.
isobutyrate + NH3
-
-
-
?
3.5.1.4
isonicotinamide + H2O
-
733190
Streptomyces sp.
isonicotinate + NH3
-
-
-
?
3.5.1.4
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows aryl acylamide hydrolase activity, EC 3.5.1.13, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with L-glutamine and L-asparagine
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
reaction of EC 3.5.1.19, nicotinamidase
733190
Streptomyces sp.
nicotinate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
733190
Streptomyces sp.
propionate + NH3
-
-
-
?
3.5.1.4
pyrazinamide + H2O
-
733190
Streptomyces sp.
pyrazinate + NH3
-
-
-
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
-
733190
Streptomyces sp. 211726
?
-
-
-
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp.
3,4-dichloroaniline + propionate
-
-
-
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp. 211726
3,4-dichloroaniline + propionate
-
-
-
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp.
?
-
-
-
-
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp. 211726
?
-
-
-
-
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp. 211726
?
-
-
-
-
3.5.1.13
p-acetotoluidide + H2O
-
733190
Streptomyces sp.
?
-
-
-
?
3.5.1.13
p-acetotoluidide + H2O
-
733190
Streptomyces sp. 211726
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
40
-
-
Streptomyces sp.
3.5.1.13
40
-
-
Streptomyces sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
15
60
activity range
Streptomyces sp.
3.5.1.13
15
60
activity range
Streptomyces sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
40
-
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.4
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.4
60
-
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
3.5.1.13
40
-
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.13
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.13
60
-
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
5.6
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
28.6
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
29.9
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
55.6
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
8
-
-
Streptomyces sp.
3.5.1.13
8
-
-
Streptomyces sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
5.5
10
activity range
Streptomyces sp.
3.5.1.13
5.5
10
activity range
Streptomyces sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.4
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
3.5.1.13
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
General Information
EC Number
General Information
Commentary
Organism
3.5.1.4
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
3.5.1.13
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.4
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
3.5.1.13
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.034
-
Benzamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.042
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.64
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.76
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
24.17
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.034
-
Benzamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.042
-
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.64
-
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.76
-
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
24.17
-
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.