Data extracted from this reference:
Activating Compound
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.4
PMSF
slight activation at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
PMSF
slight activation at 1 mM
Streptomyces sp.
Cloned(Commentary)
3.5.1.4
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces sp.
3.5.1.13
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Streptomyces sp.
Engineering
3.5.1.4
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
Inhibitors
3.5.1.4
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
KM Value [mM]
3.5.1.4
2.3
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
39.4
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
45.1
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
135.6
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
Metals/Ions
3.5.1.4
additional information
no requirement for metal ions
Streptomyces sp.
3.5.1.13
additional information
no requirement for metal ions
Streptomyces sp.
Molecular Weight [Da]
3.5.1.4
31000
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
31000
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Natural Substrates/ Products (Substrates)
3.5.1.4
4-guanidinobutyramide + H2O
Streptomyces sp.
probably the natural substrate
?
?
3.5.1.13
additional information
Streptomyces sp.
4-guanidinobutyramide probably is the natural substrate
?
3.5.1.13
additional information
Streptomyces sp. 211726
4-guanidinobutyramide probably is the natural substrate
?
Organism
3.5.1.4
Streptomyces sp.
A0A088BHP3
gene azl13
3.5.1.4
Streptomyces sp. 211726
A0A088BHP3
gene azl13
3.5.1.13
Streptomyces sp.
A0A088BHP3
gene azl13
3.5.1.13
Streptomyces sp. 211726
A0A088BHP3
gene azl13
Purification (Commentary)
3.5.1.4
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
3.5.1.13
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
Source Tissue
3.5.1.4
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
3.5.1.13
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
Specific Activity [micromol/min/mg]
3.5.1.4
0.55
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.4
0.69
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.4
0.87
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 4'-methylacetanilide
Streptomyces sp.
3.5.1.13
0.55
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.13
0.69
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.13
0.87
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide
Streptomyces sp.
Substrates and Products (Substrate)
3.5.1.4
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp.
2,6-dimethylaniline + acetate
?
3.5.1.4
3',4'-dichloropropionanilide + H2O
733190
Streptomyces sp.
3,4-dichloroaniline + propanoate
?
3.5.1.4
4'-methylacetanilide + H2O
733190
Streptomyces sp.
4-methylaniline + acetate
?
3.5.1.4
4-guanidinobutyramide + H2O
best substrate
733190
Streptomyces sp.
?
?
3.5.1.4
4-guanidinobutyramide + H2O
probably the natural substrate
733190
Streptomyces sp.
?
?
3.5.1.4
acetamide + H2O
733190
Streptomyces sp.
acetate + NH3
?
3.5.1.4
benzamide + H2O
733190
Streptomyces sp.
benzoate + NH3
?
3.5.1.4
isobutyramide + H2O
733190
Streptomyces sp.
isobutyrate + NH3
?
3.5.1.4
isonicotinamide + H2O
733190
Streptomyces sp.
isonicotinate + NH3
?
3.5.1.4
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows aryl acylamide hydrolase activity, EC 3.5.1.13, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with L-glutamine and L-asparagine
733190
Streptomyces sp.
?
3.5.1.4
nicotinamide + H2O
reaction of EC 3.5.1.19, nicotinamidase
733190
Streptomyces sp.
nicotinate + NH3
?
3.5.1.4
propionamide + H2O
733190
Streptomyces sp.
propionate + NH3
?
3.5.1.4
pyrazinamide + H2O
733190
Streptomyces sp.
pyrazinate + NH3
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp.
?
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp. 211726
?
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp.
3,4-dichloroaniline + propionate
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp. 211726
3,4-dichloroaniline + propionate
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp.
?
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp.
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp. 211726
?
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp. 211726
?
3.5.1.13
p-acetotoluidide + H2O
733190
Streptomyces sp.
?
?
3.5.1.13
p-acetotoluidide + H2O
733190
Streptomyces sp. 211726
?
?
Subunits
3.5.1.4
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Synonyms
3.5.1.4
4-guanidinobutyramide hydrolase
Streptomyces sp.
3.5.1.4
Azl13
Streptomyces sp.
3.5.1.13
aryl acylamidase
Streptomyces sp.
3.5.1.13
Azl13
Streptomyces sp.
Temperature Optimum [°C]
3.5.1.4
40
Streptomyces sp.
3.5.1.13
40
Streptomyces sp.
Temperature Range [°C]
3.5.1.4
15
60
activity range
Streptomyces sp.
3.5.1.13
15
60
activity range
Streptomyces sp.
Temperature Stability [°C]
3.5.1.4
40
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.4
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.4
60
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
3.5.1.13
40
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.13
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.13
60
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
Turnover Number [1/s]
3.5.1.4
5.6
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
28.6
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
29.9
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
55.6
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
pH Optimum
3.5.1.4
8
Streptomyces sp.
3.5.1.13
8
Streptomyces sp.
pH Range
3.5.1.4
5.5
10
activity range
Streptomyces sp.
3.5.1.13
5.5
10
activity range
Streptomyces sp.
pH Stability
3.5.1.4
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
3.5.1.13
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
Activating Compound (protein specific)
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.4
PMSF
slight activation at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
PMSF
slight activation at 1 mM
Streptomyces sp.
Cloned(Commentary) (protein specific)
3.5.1.4
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces sp.
3.5.1.13
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Streptomyces sp.
Engineering (protein specific)
3.5.1.4
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.4
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
C141A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
E41A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
3.5.1.13
K107A
site-directed mutagenesis, inactive mutant
Streptomyces sp.
Inhibitors (protein specific)
3.5.1.4
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.4
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
3.5.1.13
Ag+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cd2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Cu2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
Hg2+
complete inhibition at 1 mM
Streptomyces sp.
3.5.1.13
additional information
no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol
Streptomyces sp.
KM Value [mM] (protein specific)
3.5.1.4
2.3
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
39.4
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
45.1
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
135.6
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
Metals/Ions (protein specific)
3.5.1.4
additional information
no requirement for metal ions
Streptomyces sp.
3.5.1.13
additional information
no requirement for metal ions
Streptomyces sp.
Molecular Weight [Da] (protein specific)
3.5.1.4
31000
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
31000
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
3.5.1.4
4-guanidinobutyramide + H2O
Streptomyces sp.
probably the natural substrate
?
?
3.5.1.13
additional information
Streptomyces sp.
4-guanidinobutyramide probably is the natural substrate
?
3.5.1.13
additional information
Streptomyces sp. 211726
4-guanidinobutyramide probably is the natural substrate
?
Purification (Commentary) (protein specific)
3.5.1.4
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
3.5.1.13
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Streptomyces sp.
Source Tissue (protein specific)
3.5.1.4
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
3.5.1.13
additional information
halotolerant organism from mangrove swamps
Streptomyces sp.
Specific Activity [micromol/min/mg] (protein specific)
3.5.1.4
0.55
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.4
0.69
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.4
0.87
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 4'-methylacetanilide
Streptomyces sp.
3.5.1.13
0.55
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
Streptomyces sp.
3.5.1.13
0.69
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
Streptomyces sp.
3.5.1.13
0.87
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
3.5.1.4
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp.
2,6-dimethylaniline + acetate
?
3.5.1.4
3',4'-dichloropropionanilide + H2O
733190
Streptomyces sp.
3,4-dichloroaniline + propanoate
?
3.5.1.4
4'-methylacetanilide + H2O
733190
Streptomyces sp.
4-methylaniline + acetate
?
3.5.1.4
4-guanidinobutyramide + H2O
best substrate
733190
Streptomyces sp.
?
?
3.5.1.4
4-guanidinobutyramide + H2O
probably the natural substrate
733190
Streptomyces sp.
?
?
3.5.1.4
acetamide + H2O
733190
Streptomyces sp.
acetate + NH3
?
3.5.1.4
benzamide + H2O
733190
Streptomyces sp.
benzoate + NH3
?
3.5.1.4
isobutyramide + H2O
733190
Streptomyces sp.
isobutyrate + NH3
?
3.5.1.4
isonicotinamide + H2O
733190
Streptomyces sp.
isonicotinate + NH3
?
3.5.1.4
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows aryl acylamide hydrolase activity, EC 3.5.1.13, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with L-glutamine and L-asparagine
733190
Streptomyces sp.
?
3.5.1.4
nicotinamide + H2O
reaction of EC 3.5.1.19, nicotinamidase
733190
Streptomyces sp.
nicotinate + NH3
?
3.5.1.4
propionamide + H2O
733190
Streptomyces sp.
propionate + NH3
?
3.5.1.4
pyrazinamide + H2O
733190
Streptomyces sp.
pyrazinate + NH3
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp.
?
?
3.5.1.13
2',6'-dimethylacetanilide + H2O
733190
Streptomyces sp. 211726
?
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp.
3,4-dichloroaniline + propionate
?
3.5.1.13
3',4'-dichloropropionanilide + H2O
i.e. propanil
733190
Streptomyces sp. 211726
3,4-dichloroaniline + propionate
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp.
?
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp.
?
3.5.1.13
additional information
4-guanidinobutyramide probably is the natural substrate
733190
Streptomyces sp. 211726
?
3.5.1.13
additional information
substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine
733190
Streptomyces sp. 211726
?
3.5.1.13
p-acetotoluidide + H2O
733190
Streptomyces sp.
?
?
3.5.1.13
p-acetotoluidide + H2O
733190
Streptomyces sp. 211726
?
?
Subunits (protein specific)
3.5.1.4
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
3.5.1.13
?
x * 31000, recombinant His-tagged enzyme, SDS-PAGE
Streptomyces sp.
Temperature Optimum [°C] (protein specific)
3.5.1.4
40
Streptomyces sp.
3.5.1.13
40
Streptomyces sp.
Temperature Range [°C] (protein specific)
3.5.1.4
15
60
activity range
Streptomyces sp.
3.5.1.13
15
60
activity range
Streptomyces sp.
Temperature Stability [°C] (protein specific)
3.5.1.4
40
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.4
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.4
60
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
3.5.1.13
40
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
Streptomyces sp.
3.5.1.13
45
50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
Streptomyces sp.
3.5.1.13
60
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation
Streptomyces sp.
Turnover Number [1/s] (protein specific)
3.5.1.4
5.6
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
28.6
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
29.9
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
55.6
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
pH Optimum (protein specific)
3.5.1.4
8
Streptomyces sp.
3.5.1.13
8
Streptomyces sp.
pH Range (protein specific)
3.5.1.4
5.5
10
activity range
Streptomyces sp.
3.5.1.13
5.5
10
activity range
Streptomyces sp.
pH Stability (protein specific)
3.5.1.4
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
3.5.1.13
4.5
10
purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h
Streptomyces sp.
General Information
3.5.1.4
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
3.5.1.13
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
General Information (protein specific)
3.5.1.4
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
3.5.1.13
evolution
the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases
Streptomyces sp.
KCat/KM [mM/s]
3.5.1.4
0.034
Benzamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.042
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.64
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.76
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
24.17
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
KCat/KM [mM/s] (protein specific)
3.5.1.4
0.034
Benzamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.042
nicotinamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.64
Propionamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
0.76
acetamide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.
3.5.1.4
24.17
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
Streptomyces sp.