BRENDA - Enzyme Database

Revealing the complementation of ferredoxin by cytochrome b5 in the Spirulina-DELTA6-desaturation reaction by N-terminal fusion and co-expression of the fungal-cytochrome b5 domain and Spirulina-DELTA6-acyl-lipid desaturase

Hongsthong, A.; Subudhi, S.; Sirijuntarut, M.; Kurdrid, P.; Cheevadhanarak, S.; Tanticharoen, M.; Appl. Microbiol. Biotechnol. 72, 1192-1201 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.46
expressed as N-terminally fused and co-expressed protein with the cytochrome b5 domain from Mucor rouxii, in Escherichia coli DH5alpha cells and Saccharomyces cerevisiae strain DBY746
Arthrospira platensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.46
50000
-
x * 50000, SDS-PAGE
Arthrospira platensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.46
1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arthrospira platensis
-
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.46
Arthrospira platensis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.46
1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733181
Arthrospira platensis
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.46
?
x * 50000, SDS-PAGE
Arthrospira platensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.46
Ferredoxin
-
Arthrospira platensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.46
expressed as N-terminally fused and co-expressed protein with the cytochrome b5 domain from Mucor rouxii, in Escherichia coli DH5alpha cells and Saccharomyces cerevisiae strain DBY746
Arthrospira platensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.46
Ferredoxin
-
Arthrospira platensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.46
50000
-
x * 50000, SDS-PAGE
Arthrospira platensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.46
1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arthrospira platensis
-
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.46
1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733181
Arthrospira platensis
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.46
?
x * 50000, SDS-PAGE
Arthrospira platensis