Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mahan, K.M.; Penrod, J.T.; Ju, K.S.; Al Kass, N.; Tan, W.A.; Truong, R.; Parales, J.V.; Parales, R.E.
    Selection for growth on 3-nitrotoluene by 2-nitrotoluene-utilizing Acidovorax sp. strain JS42 identifies nitroarene dioxygenases with altered specificities (2015), Appl. Environ. Microbiol., 81, 309-319.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.23 A405G synthesizes 17% 3-methylcatechol, 83% 4-methylcatechol from substrate 3-nitrotoluene Acidovorax sp.
1.14.12.23 I204A synthesizes 2% 3-methylcatechol, 97% 4-methylcatechol and 1% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.
1.14.12.23 I204T synthesizes 5% 3-methylcatechol, 93% 4-methylcatechol and 2% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.
1.14.12.23 I204V synthesizes 13% 3-methylcatechol, 83% 4-methylcatechol and 5% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.23 0.0012
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.003
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0038
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0039
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0042
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0045
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0047
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0048
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0054
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0057
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0062
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0078
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0092
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0099
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.0137
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.019
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.04
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.047
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.23 Acidovorax sp.
-
-
-
1.14.12.23 Acidovorax sp. JS42
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.23 2-nitrotoluene + NADH + O2
-
 Acidovorax sp. 3-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 2-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 3-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 3-nitrotoluene + NADH + O2
-
 Acidovorax sp. 3-methylcatechol + nitrite + NAD+ products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol ?
1.14.12.23 3-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 3-methylcatechol + nitrite + NAD+ products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol ?
1.14.12.23 3-nitrotoluene + NADH + O2
-
 Acidovorax sp. 4-methylcatechol + nitrite + NAD+ sole product ?
1.14.12.23 3-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 4-methylcatechol + nitrite + NAD+ sole product ?
1.14.12.23 4-nitrotoluene + NADH + O2
-
 Acidovorax sp. 4-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 4-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 4-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 additional information all three mononitrotoluene isomers serve as substrates for 2-nitrotoluene dioxygenase, but strain JS42 is unable to grow on 3- or 4-nitrotoluene Acidovorax sp. ?
-
 ?
1.14.12.23 nitrobenzene + NADH + O2
-
 Acidovorax sp. catechol + nitrite + NAD+
-
 ?
1.14.12.23 nitrobenzene + NADH + O2
-
 Acidovorax sp. catechol + nitrite + NAD+ reaction of EC 1.14.12.23 ?
1.14.12.23 nitrobenzene + NADH + O2
-
 Acidovorax sp. JS42 catechol + nitrite + NAD+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.23 0.12
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.13
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.15
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.16
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.19
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.19
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.21
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.21
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.22
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.23
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.25
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.25
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.26
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.27
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.27
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.27
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.3
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.34
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.

General Information

EC Number General Information Comment Organism
1.14.12.23 physiological function Acidovorax sp. strain JS42 uses 2-nitrotoluene as a sole source of carbon and energy. The first enzyme of the degradation pathway, 2-nitrotoluene 2,3-dioxygenase, adds both atoms of molecular oxygen to 2-nitrotoluene, forming nitrite and 3-methylcatechol Acidovorax sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.12.23 3.7
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 4
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 6.5
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 12
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 17
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 22
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 29
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 37
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 37
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 39
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 53
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 58
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 65
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 67
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 68
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 83
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 90
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
1.14.12.23 160
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.