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Literature summary extracted from

  • Huang, F.C.; Peter, A.; Schwab, W.
    Expression and characterization of CYP52 genes involved in the biosynthesis of sophorolipid and alkane metabolism from Starmerella bombicola (2014), Appl. Environ. Microbiol., 80, 766-776.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.80 0.04
-
 oleic acid pH 7.0, 30°C Starmerella bombicola
1.14.14.80 0.054
-
 arachidonic acid pH 7.0, 30°C Starmerella bombicola
1.14.14.80 0.068
-
 linoleic acid pH 7.0, 30°C Starmerella bombicola

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.80 Starmerella bombicola B8QHP1
-
-
1.14.14.80 Starmerella bombicola B8QHP3
-
-
1.14.14.80 Starmerella bombicola B8QHP5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.80 alpha-linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 additional information isoform CYP52M1 oxidizes C16 to C20 fatty acids preferentially. It converts oleic acid (C18:1) more efficiently than stearic acid (C18:0) and linoleic acid (C18:2) and much more effectively than alpha-linolenic acid (C18:3). No products are detected when C10 to C12 fatty acids are used as the substrates. CYP52M1 hydroxylates fatty acids at their omega- and omega-1 positions. No activity is detected toward short-chain fatty acids (C10 to C14), cis-11-eicosenoic acid (C20:1), and C16 to C18 alkanes Starmerella bombicola ?
-
 ?
1.14.14.80 additional information isoform CYP52N1 oxidizes C14 to C20 saturated and unsaturated fatty acids and preferentially oxidizes palmitic acid, oleic acid, and linoleic acid. It only catalyzes omega-hydroxylation of fatty acids Starmerella bombicola ?
-
 ?
1.14.14.80 additional information isoform CYP52N1 shows minor omega-hydroxylation activity against myristic acid, palmitic acid, palmitoleic acid, and oleic acid Starmerella bombicola ?
-
 ?
1.14.14.80 myristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Starmerella bombicola 18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.80 CYP52-E3
-
 Starmerella bombicola
1.14.14.80 CYP52-M1
-
 Starmerella bombicola
1.14.14.80 CYP52-N1
-
 Starmerella bombicola
1.14.14.80 CYP52E3
-
 Starmerella bombicola
1.14.14.80 CYP52M1
-
 Starmerella bombicola
1.14.14.80 CYP52N1
-
 Starmerella bombicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.80 25 30
-
 Starmerella bombicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.80 7
-
-
 Starmerella bombicola

General Information

EC Number General Information Comment Organism
1.14.14.80 physiological function isoform CYP52M1 is involved in the biosynthesis of sophorolipid Starmerella bombicola