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Literature summary extracted from

  • Ohbayashi, N.; Yamagata, N.; Goto, M.; Watanabe, K.; Yamagata, Y.; Murayama, K.
    Enhancement of the structural stability of full-length clostridial collagenase by calcium ions (2012), Appl. Environ. Microbiol., 78, 5839-5844.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.3 expression in Escherichia coli Hathewaya histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.3 EGTA Ca2+ chelation by EGTA induces interdomain conformational changes with an increase in the percent polydispersity as the Ca2+ is chelated, suggesting an increase in protein flexibility. The thermostability is decreased by Ca2+ chelation Hathewaya histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.3 Ca2+ interdomain flexibility and the domain arrangement of full-length collagenase H are reversibly regulated by Ca2+ Hathewaya histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.3 82000
-
 gel filtration Hathewaya histolytica
3.4.24.3 114000
-
 1 * 114000, calculated Hathewaya histolytica
3.4.24.3 119000
-
 gel filtration, presence of EGTA Hathewaya histolytica

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.3 Hathewaya histolytica Q46085
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.3 azokoll + H2O
-
 Hathewaya histolytica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.3 monomer 1 * 114000, calculated Hathewaya histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.24.3 ColH
-
 Hathewaya histolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.3 49
-
 melting point, presence of EGTA. Addition of excess Ca2+ restores melting point to 54°C Hathewaya histolytica
3.4.24.3 54
-
 melting point Hathewaya histolytica