EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.3 | expression in Escherichia coli | Hathewaya histolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.3 | EGTA | Ca2+ chelation by EGTA induces interdomain conformational changes with an increase in the percent polydispersity as the Ca2+ is chelated, suggesting an increase in protein flexibility. The thermostability is decreased by Ca2+ chelation | Hathewaya histolytica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.3 | Ca2+ | interdomain flexibility and the domain arrangement of full-length collagenase H are reversibly regulated by Ca2+ | Hathewaya histolytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.3 | 82000 | - |
gel filtration | Hathewaya histolytica |
3.4.24.3 | 114000 | - |
1 * 114000, calculated | Hathewaya histolytica |
3.4.24.3 | 119000 | - |
gel filtration, presence of EGTA | Hathewaya histolytica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.3 | Hathewaya histolytica | Q46085 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.3 | azokoll + H2O | - |
Hathewaya histolytica | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.3 | monomer | 1 * 114000, calculated | Hathewaya histolytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.3 | ColH | - |
Hathewaya histolytica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.3 | 49 | - |
melting point, presence of EGTA. Addition of excess Ca2+ restores melting point to 54°C | Hathewaya histolytica |
3.4.24.3 | 54 | - |
melting point | Hathewaya histolytica |