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Literature summary extracted from

  • Gao, L.; Danno, A.; Fujii, S.; Fukuda, W.; Imanaka, T.; Fujiwara, S.
    Indole-3-glycerol-phosphate synthase is recognized by a cold-inducible group II chaperonin in Thermococcus kodakarensis (2012), Appl. Environ. Microbiol., 78, 3806-3815.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.B10 gene cpkA, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli Thermococcus kodakarensis
3.6.4.B10 gene cpkB, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.B10 additional information construction of gene cpkA disruption mutant strains. Gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview Thermococcus kodakarensis
3.6.4.B10 additional information construction of gene cpkB disruption mutant strains. Gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview Thermococcus kodakarensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.4.B10 cytoplasm
-
Thermococcus kodakarensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B10 Mg2+ required Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.4.B10 60000
-
x * 60000, about, CpkA, SDS-PAGE Thermococcus kodakarensis
3.6.4.B10 60000
-
x * 60000, about, CpkB, SDS-PAGE Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B10 ATP + H2O Thermococcus kodakarensis
-
ADP + phosphate
-
?
3.6.4.B10 additional information Thermococcus kodakarensis indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA ?
-
?
3.6.4.B10 additional information Thermococcus kodakarensis indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B10 Thermococcus kodakarensis P61111 subunit alpha; cold-inducible CpkA
-
3.6.4.B10 Thermococcus kodakarensis Q52500 subunit beta; heat-inducible CpkB
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.4.B10 additional information Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C Thermococcus kodakarensis
-
3.6.4.B10 additional information Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C Thermococcus kodakarensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B10 ATP + H2O
-
Thermococcus kodakarensis ADP + phosphate
-
?
3.6.4.B10 additional information indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA Thermococcus kodakarensis ?
-
?
3.6.4.B10 additional information indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB Thermococcus kodakarensis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.6.4.B10 ? x * 60000, about, CpkA, SDS-PAGE Thermococcus kodakarensis
3.6.4.B10 ? x * 60000, about, CpkB, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B10 cPKA
-
Thermococcus kodakarensis
3.6.4.B10 CpkB
-
Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B10 7.5
-
ATPase assay at Thermococcus kodakarensis
3.6.4.B10 8
-
chaperonin assay at Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.6.4.B10 malfunction gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C, DA1 cells grow optimally in minimal medium only in the presence of tryptophan but hardly grow in the absence of tryptophan at 60°C. A lesion of functional indole-3-glycerol-phosphate synthase, TrpC, is caused by cpkA disruption, resulting in tryptophan auxotrophy Thermococcus kodakarensis
3.6.4.B10 malfunction gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C Thermococcus kodakarensis
3.6.4.B10 physiological function the CpkA C-terminal region plays a key role in cell growth at 60°C Thermococcus kodakarensis