EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.4.B10 | gene cpkA, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli | Thermococcus kodakarensis |
3.6.4.B10 | gene cpkB, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli | Thermococcus kodakarensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.4.B10 | additional information | construction of gene cpkA disruption mutant strains. Gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview | Thermococcus kodakarensis |
3.6.4.B10 | additional information | construction of gene cpkB disruption mutant strains. Gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview | Thermococcus kodakarensis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.4.B10 | cytoplasm | - |
Thermococcus kodakarensis | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.4.B10 | Mg2+ | required | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.4.B10 | 60000 | - |
x * 60000, about, CpkA, SDS-PAGE | Thermococcus kodakarensis |
3.6.4.B10 | 60000 | - |
x * 60000, about, CpkB, SDS-PAGE | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.B10 | ATP + H2O | Thermococcus kodakarensis | - |
ADP + phosphate | - |
? | |
3.6.4.B10 | additional information | Thermococcus kodakarensis | indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA | ? | - |
? | |
3.6.4.B10 | additional information | Thermococcus kodakarensis | indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.4.B10 | Thermococcus kodakarensis | P61111 | subunit alpha; cold-inducible CpkA | - |
3.6.4.B10 | Thermococcus kodakarensis | Q52500 | subunit beta; heat-inducible CpkB | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.4.B10 | additional information | Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C | Thermococcus kodakarensis | - |
3.6.4.B10 | additional information | Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C | Thermococcus kodakarensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.B10 | ATP + H2O | - |
Thermococcus kodakarensis | ADP + phosphate | - |
? | |
3.6.4.B10 | additional information | indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA | Thermococcus kodakarensis | ? | - |
? | |
3.6.4.B10 | additional information | indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB | Thermococcus kodakarensis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.4.B10 | ? | x * 60000, about, CpkA, SDS-PAGE | Thermococcus kodakarensis |
3.6.4.B10 | ? | x * 60000, about, CpkB, SDS-PAGE | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.4.B10 | cPKA | - |
Thermococcus kodakarensis |
3.6.4.B10 | CpkB | - |
Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.4.B10 | 7.5 | - |
ATPase assay at | Thermococcus kodakarensis |
3.6.4.B10 | 8 | - |
chaperonin assay at | Thermococcus kodakarensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.4.B10 | malfunction | gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C, DA1 cells grow optimally in minimal medium only in the presence of tryptophan but hardly grow in the absence of tryptophan at 60°C. A lesion of functional indole-3-glycerol-phosphate synthase, TrpC, is caused by cpkA disruption, resulting in tryptophan auxotrophy | Thermococcus kodakarensis |
3.6.4.B10 | malfunction | gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C | Thermococcus kodakarensis |
3.6.4.B10 | physiological function | the CpkA C-terminal region plays a key role in cell growth at 60°C | Thermococcus kodakarensis |