BRENDA - Enzyme Database

Purification, characterization, and crystallization of the components of the nitrobenzene and 2-nitrotoluene dioxygenase enzyme systems

Parales, R.; Huang, R.; Yu, C.; Parales, J.; Lee, F.; Lessner, D.; Ivkovic-Jensen, M.; Liu, W.; Friemann, R.; Ramaswamy, S.; Gibson, D.; Appl. Environ. Microbiol. 71, 3806-3814 (2005)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.14.12.23
oxygenase component, to 1.2 A resolution, hexagonal space group P63 with cell dimensions a = b = 121.6 A and c = 84.4 A
Acidovorax sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.12.23
0.02
-
2-nitrotoluene
pH 6.8, 30°C
Acidovorax sp.
1.14.12.23
0.121
-
Naphthalene
pH 6.8, 30°C
Acidovorax sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.12.23
25300
-
3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
25500
-
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
50400
-
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
51200
-
3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
210000
-
gel filtration, oxygenase component
Acidovorax sp.
1.14.12.23
215900
-
dynamic light scattering, oxygenase component
Acidovorax sp.
1.14.12.23
216000
-
gel filtration, oxygenase component
Acidovorax sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.12.23
Acidovorax sp.
-
-
-
1.14.12.23
Acidovorax sp. JS42
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.12.23
2-nitrotoluene + NADH + O2
-
733153
Acidovorax sp.
3-methylcatechol + nitrite + NAD+
-
-
-
?
1.14.12.23
2-nitrotoluene + NADH + O2
-
733153
Acidovorax sp. JS42
3-methylcatechol + nitrite + NAD+
-
-
-
?
1.14.12.23
naphthalene + NADH + O2
-
733153
Acidovorax sp.
? + nitrite + NAD+
-
-
-
?
1.14.12.23
naphthalene + NADH + O2
-
733153
Acidovorax sp. JS42
? + nitrite + NAD+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.12.23
hexamer
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component; 3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.12.23
2.3
-
2-nitrotoluene
pH 6.8, 30°C
Acidovorax sp.
1.14.12.23
2.4
-
Naphthalene
pH 6.8, 30°C
Acidovorax sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.12.23
NADH
0.5 mol of NADH is required to reduce each alphabeta heterodimer of oxygenase
Acidovorax sp.
1.14.12.23
[2Fe-2S]-center
presence of a Rieske [2Fe-2S] cluster in the oxygenase component
Acidovorax sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.12.23
NADH
0.5 mol of NADH is required to reduce each alphabeta heterodimer of oxygenase
Acidovorax sp.
1.14.12.23
[2Fe-2S]-center
presence of a Rieske [2Fe-2S] cluster in the oxygenase component
Acidovorax sp.
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.14.12.23
oxygenase component, to 1.2 A resolution, hexagonal space group P63 with cell dimensions a = b = 121.6 A and c = 84.4 A
Acidovorax sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.12.23
0.02
-
2-nitrotoluene
pH 6.8, 30°C
Acidovorax sp.
1.14.12.23
0.121
-
Naphthalene
pH 6.8, 30°C
Acidovorax sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.12.23
25300
-
3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
25500
-
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
50400
-
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
51200
-
3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
1.14.12.23
210000
-
gel filtration, oxygenase component
Acidovorax sp.
1.14.12.23
215900
-
dynamic light scattering, oxygenase component
Acidovorax sp.
1.14.12.23
216000
-
gel filtration, oxygenase component
Acidovorax sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.12.23
2-nitrotoluene + NADH + O2
-
733153
Acidovorax sp.
3-methylcatechol + nitrite + NAD+
-
-
-
?
1.14.12.23
2-nitrotoluene + NADH + O2
-
733153
Acidovorax sp. JS42
3-methylcatechol + nitrite + NAD+
-
-
-
?
1.14.12.23
naphthalene + NADH + O2
-
733153
Acidovorax sp.
? + nitrite + NAD+
-
-
-
?
1.14.12.23
naphthalene + NADH + O2
-
733153
Acidovorax sp. JS42
? + nitrite + NAD+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.12.23
hexamer
3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component; 3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component
Acidovorax sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.12.23
2.3
-
2-nitrotoluene
pH 6.8, 30°C
Acidovorax sp.
1.14.12.23
2.4
-
Naphthalene
pH 6.8, 30°C
Acidovorax sp.