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Literature summary extracted from

  • Lessner, D.; Johnson, G.; Parales, R.; Spain, J.; Gibson, D.
    Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765 (2002), Appl. Environ. Microbiol., 68, 634-641.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.23 Comamonas sp. P95561 and Q8RTL5 and Q8RTL4 and Q8RTL3 P95561 i.e.subunit nbzAa, reductase NBDR, Q8RTL5 i.e. ferredoxin, subunit nbzAb, Q8RTL4 i.e. oxygenase alpha-subunit nbzAc, NBDO, Q8RTL3 i.e. oxygenase beta-subunit nbzAd, NBDO
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.23 1,3-dinitrobenzene + NADH + O2
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Comamonas sp. 4-nitrocatechol + nitrite + NAD+ 100% yield. 89% of the activity with ntrobenzene ?
1.14.12.23 2-nitrotoluene + NADH + O2
-
Comamonas sp. 3-methylcatechol + nitrite + NAD+ 55% yield, plus 45% 2-nitrobenzyl alcohol. 59% of the activity with ntrobenzene ?
1.14.12.23 2-nitrotoluene + NADH + O2
-
Comamonas sp. 4-methylcatechol + nitrite + NAD+ 100% yield. 221% of the activity with ntrobenzene ?
1.14.12.23 4-nitrotoluene + NADH + O2
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Comamonas sp. 4-methylcatechol + nitrite + NAD+ 85% yield, plus 15% 4-nitrobenzyl alcohol. 42% of the activity with ntrobenzene ?
1.14.12.23 additional information a nitro-substituted position is generally the preferred, but not exclusive, oxidation site. All of the nitrotoluene and dinitrotoluene isomers tested are transformed. Oxidation at the 3,4 position of the ring, with respect to the methyl substituent, appears to be favored. The nitro substituent appears to contribute significantly to specificity Comamonas sp. ?
-
?
1.14.12.23 naphthalene + NADH + O2
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Comamonas sp. 1,2-dihydronaphthalene cis-1,2-diol + NAD+ 100% yield. 59% of the activity with ntrobenzene ?
1.14.12.23 nitrobenzene + NADH + O2
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Comamonas sp. catechol + nitrite + NAD+ 100% yield ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.23 nbzAa
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Comamonas sp.
1.14.12.23 nbzAb
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Comamonas sp.
1.14.12.23 nbzAc
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Comamonas sp.
1.14.12.23 nbzAd
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Comamonas sp.