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Literature summary extracted from

  • Sudhir, A.P.; Dave, B.R.; Prajapati, A.S.; Panchal, K.; Patel, D.; Subramanian, R.B.
    Characterization of a recombinant glutaminase-free L-asparaginase (ansA3) enzyme with high catalytic activity from Bacillus licheniformis (2014), Appl. Biochem. Biotechnol., 174, 2504-2515.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Ca2+ about 70% residual activity at 5 mM Bacillus licheniformis
3.5.1.1 EDTA less than 60% residual activity at 5 mM Bacillus licheniformis
3.5.1.1 Fe3+ less than 60% residual activity at 5 mM Bacillus licheniformis
3.5.1.1 Hg2+ complete inhibition at 5 mM Bacillus licheniformis
3.5.1.1 K+ less than 60% residual activity at 5 mM Bacillus licheniformis
3.5.1.1 Ni2+ about 85% residual activity at 5 mM Bacillus licheniformis
3.5.1.1 Urea less than 60% residual activity at 5 mM Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.671
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ slightly increased activity at 5 mM Bacillus licheniformis
3.5.1.1 Mn2+ slightly increased activity at 5 mM Bacillus licheniformis
3.5.1.1 Na+ slightly increased activity at 5 mM Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 37000
-
 1 * 37000, SDS-PAGE Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Bacillus licheniformis 100% activity L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Bacillus licheniformis MTCC 429 100% activity L-aspartate + NH3
-
 ?
3.5.1.1 L-aspartic acid + NH3 Bacillus licheniformis less than 3% activity compared to L-asparagine ?
-
 ?
3.5.1.1 L-aspartic acid + NH3 Bacillus licheniformis MTCC 429 less than 3% activity compared to L-asparagine ?
-
 ?
3.5.1.1 L-glutamine + H2O Bacillus licheniformis less than 1% activity compared to L-asparagine L-glutamate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O Bacillus licheniformis MTCC 429 less than 1% activity compared to L-asparagine L-glutamate + NH3
-
 ?
3.5.1.1 additional information Bacillus licheniformis no activity with D-asparagine monohydrate and L-glutamic acid ?
-
 ?
3.5.1.1 additional information Bacillus licheniformis MTCC 429 no activity with D-asparagine monohydrate and L-glutamic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Bacillus licheniformis R4L284
-
-
3.5.1.1 Bacillus licheniformis MTCC 429 R4L284
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 Ni-NTA column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 135
-
 crude extract, at 37°C and pH 8.0 Bacillus licheniformis
3.5.1.1 407.6
-
 after 3.02fold purification, at 37°C and pH 8.0 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O 100% activity Bacillus licheniformis L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O 100% activity Bacillus licheniformis MTCC 429 L-aspartate + NH3
-
 ?
3.5.1.1 L-aspartic acid + NH3 less than 3% activity compared to L-asparagine Bacillus licheniformis ?
-
 ?
3.5.1.1 L-aspartic acid + NH3 less than 3% activity compared to L-asparagine Bacillus licheniformis MTCC 429 ?
-
 ?
3.5.1.1 L-glutamine + H2O less than 1% activity compared to L-asparagine Bacillus licheniformis L-glutamate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O less than 1% activity compared to L-asparagine Bacillus licheniformis MTCC 429 L-glutamate + NH3
-
 ?
3.5.1.1 additional information no activity with D-asparagine monohydrate and L-glutamic acid Bacillus licheniformis ?
-
 ?
3.5.1.1 additional information no activity with D-asparagine monohydrate and L-glutamic acid Bacillus licheniformis MTCC 429 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 monomer 1 * 37000, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ansA3
-
 Bacillus licheniformis
3.5.1.1 L-asparaginase III isoform Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 36.8
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 7 9 more than 50% activity between pH 7.0 and 9.0, the enzyme is inactive at pH 6.0 and 10.0 Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 54
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis