Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhou, N.D.; Gu, X.L.; Tian, Y.P.
    Isolation and characterization of urethanase from Penicillium variabile and its application to reduce ethyl carbamate contamination in Chinese rice wine (2013), Appl. Biochem. Biotechnol., 170, 718-728.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.75 food industry with good ethanol tolerance, the crude urethanase is able to reduce ethyl carbamate, i.e. urethane, in Chinese rice wine without the change of flavor substance in wine Talaromyces variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.75 urethane + H2O Talaromyces variabilis
-
 ethanol + CO2 + NH3
-
 ?
3.5.1.75 urethane + H2O Talaromyces variabilis JN-A525
-
 ethanol + CO2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.75 Talaromyces variabilis
-
 isolated from mouse gastrointestine
-
3.5.1.75 Talaromyces variabilis JN-A525
-
 isolated from mouse gastrointestine
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.75 the enzyme exhibits ethanol tolerance, 50% activity remaining at 27% ethanol, 65% activity remaining at 15% ethanol Talaromyces variabilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.75 mycelium
-
 Talaromyces variabilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.75 methyl carbamate + H2O 20% of the activity with ethyl carbamate, i.e. urethane Talaromyces variabilis methanol + CO2 + NH3
-
 ?
3.5.1.75 methyl carbamate + H2O 20% of the activity with ethyl carbamate, i.e. urethane Talaromyces variabilis JN-A525 methanol + CO2 + NH3
-
 ?
3.5.1.75 additional information no or poor activity with gamma-aminobutyric acid, glutamic acid, and glycine Talaromyces variabilis ?
-
 ?
3.5.1.75 additional information no or poor activity with gamma-aminobutyric acid, glutamic acid, and glycine Talaromyces variabilis JN-A525 ?
-
 ?
3.5.1.75 urethane + H2O
-
 Talaromyces variabilis ethanol + CO2 + NH3
-
 ?
3.5.1.75 urethane + H2O
-
 Talaromyces variabilis JN-A525 ethanol + CO2 + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.75 50
-
-
 Talaromyces variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.75 50
-
 stable up to Talaromyces variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.75 6
-
-
 Talaromyces variabilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.75 3 10 activity range, profile overview Talaromyces variabilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.75 1 2 1 h, 4°C, 50% of maximal activity remaining Talaromyces variabilis
3.5.1.75 5
-
 1 h, 4°C, 50% of maximal activity remaining, Talaromyces variabilis
3.5.1.75 7 10 stable at, 1 h, 4°C, over 80% activity remaining Talaromyces variabilis

General Information

EC Number General Information Comment Organism
3.5.1.75 physiological function urethanase can directly catalyze the decomposition of urethane, which has potential carcinogenic and teratogenic effects and exists widely in various fermented foods and alcoholic beverages Talaromyces variabilis