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Literature summary extracted from

  • Lu, H.; Gu, M.; Huang, Q.; Huang, J.; Lu, W.; Lu, H.; Huang, Q.
    Hydrogen/deuterium exchange mass spectrometry and site-directed disulfide cross-linking suggest an important dynamic interface between the two lysostaphin domains (2013), Antimicrob. Agents Chemother., 57, 1872-1881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.75 expressed in Escherichia coli BL21(DE3) cells Staphylococcus simulans

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.75 D339C the mutant shows about 37% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 K332C the mutant shows about 82% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 K332C/T464C the mutant retains around 25% of the bacteriolytic activity of the wild type protein Staphylococcus simulans
3.4.24.75 K336C the mutant shows about 90% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 L258C the mutant shows about 35% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 N260C the mutant shows about 80% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 Q256C the mutant shows about 85% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 R470C the mutant shows about 50% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 T464C the mutant shows about 85% of wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 T477C the mutant shows wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 T482C the mutant shows wild type bacteriolytic activity Staphylococcus simulans
3.4.24.75 V487C the mutant shows wild type bacteriolytic activity Staphylococcus simulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.75 additional information the bacteriolytic activity of the enzyme is reduced to about 60% in the presence of 150 mM NaCl which causes contractions of bacterial peptidoglycan substrate Staphylococcus simulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.75 peptidoglycan + H2O Staphylococcus simulans
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.75 Staphylococcus simulans P10547
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.75 Capto MMC column chromatography, and Sephadex G-50 gel filtration Staphylococcus simulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.75 peptidoglycan + H2O
-
 Staphylococcus simulans ?
-
 ?