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Literature summary extracted from

  • Kim, C.J.; Lee, D.I.; Lee, C.H.; Ahn, I.S.
    A dityrosine-based substrate for a protease assay: application for the selective assessment of papain and chymopapain activity (2012), Anal. Chim. Acta, 723, 101-107.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.00691
-
 N,N-diBoc-dityrosine-(isoniazid)2 pH 6.2, 25°C Carica papaya

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.2 Na+
-
 Carica papaya
3.4.22.6 Na+
-
 Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-
3.4.22.6 Carica papaya P14080
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 latex
-
 Carica papaya
-
3.4.22.6 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 N,N-diBoc-dityrosine-(isoniazid)2 + H2O
-
 Carica papaya ?
-
 ?
3.4.22.6 N,N-diBoc-dityrosine-(isoniacid)2 + H2O
-
 Carica papaya N,N-diBoc-dityrosine + 2 isoniacid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain
-
 Carica papaya
3.4.22.6 Chymopapain
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 25
-
 assay at Carica papaya
3.4.22.2 60
-
 optimal temperature Carica papaya
3.4.22.6 25
-
 assay at Carica papaya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.2 80
-
 no hydrolysis at all at temperatures higher than 80°C Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6.2
-
 assay at Carica papaya
3.4.22.6 6.2
-
 assay at Carica papaya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.2 4 8
-
 Carica papaya