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Literature summary extracted from

  • Kotzsch, A.; Skovgaard, T.; Buus, U.; Andersen, S.; Devkota, K.; Berthelsen, J.
    A substrate-optimized electrophoretic mobility shift assay for ADAM12 (2014), Anal. Biochem., 452, 34-42.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B10 batimastat
-
 Homo sapiens
3.4.24.B10 GM6001
-
 Homo sapiens
3.4.24.B10 marimastat
-
 Homo sapiens
3.4.24.B10 MMP inhibitor III
-
 Homo sapiens
3.4.24.B10 MMP inhibitor V
-
 Homo sapiens
3.4.24.B10 MMP-3 inhibitor VIII
-
 Homo sapiens
3.4.24.B10 TAPI-0
-
 Homo sapiens
3.4.24.B10 TAPI-1
-
 Homo sapiens
3.4.24.B10 TAPI-2
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B10 insulin-like growth factor binding protein-3 + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B10 Homo sapiens O43184
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B10 anion exchange column chromatography and concanavalin A column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B10 HEK-293-EBNA cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B10 5-Fam-EFPIYDFLPAKKK-NH2 + H2O
-
 Homo sapiens 5-Fam-EFPI + YDFLPAKKK-NH2
-
 ?
3.4.24.B10 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 + H2O best substrate Homo sapiens 5-Fam-FFLAQA + (homophenylalanine)RSK-NH2
-
 ?
3.4.24.B10 5-Fam-HADLAQA(homophenylalanine)RSK-NH2 + H2O
-
 Homo sapiens 5-Fam-HADLAQA + (homophenylalanine)RSK-NH2
-
 ?
3.4.24.B10 5-Fam-HALAQA(homophenylalanine)RSK-NH2 + H2O
-
 Homo sapiens 5-Fam-HALAQA + (homophenylalanine)RSK-NH2
-
 ?
3.4.24.B10 5-Fam-LAQA(homophenylalanine)RSK-NH2 + H2O
-
 Homo sapiens 5-Fam-LAQA + (homophenylalanine)RSK-NH2
-
 ?
3.4.24.B10 5-Fam-SAVSRLRAYLLPA-NH2 + H2O
-
 Homo sapiens 5-Fam-SAVSRLR + AYLLPA-NH2
-
 ?
3.4.24.B10 Dabcyl-LAQA(homophenylalanine)RSK(5-FAM)-NH2 + H2O
-
 Homo sapiens Dabcyl-LAQA + (homophenylalanine)RSK(5-FAM)-NH2
-
 ?
3.4.24.B10 insulin-like growth factor binding protein-3 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B10 ADAM12
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.B10 0.000025
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens batimastat
3.4.24.B10 0.000028
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens batimastat
3.4.24.B10 0.00012
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP inhibitor V
3.4.24.B10 0.00018
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP inhibitor V
3.4.24.B10 0.00035
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-0
3.4.24.B10 0.0004
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-0
3.4.24.B10 0.00043
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens marimastat
3.4.24.B10 0.0006
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP inhibitor III
3.4.24.B10 0.00067
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens marimastat
3.4.24.B10 0.001
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-1
3.4.24.B10 0.0011
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP inhibitor III
3.4.24.B10 0.0011
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens GM6001
3.4.24.B10 0.0014
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-2
3.4.24.B10 0.0014
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-1
3.4.24.B10 0.0015
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP-3 inhibitor VIII
3.4.24.B10 0.0018
-
 with 5-Fam-HALAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens TAPI-2
3.4.24.B10 0.0021
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens GM6001
3.4.24.B10 0.0028
-
 with 5-Fam-FFLAQA(homophenylalanine)RSK-NH2 as substrate, at pH 8.0 and 22°C Homo sapiens MMP-3 inhibitor VIII