BRENDA - Enzyme Database

Porphyromonas gingivalis peptidylarginine deiminase substrate specificity

Abdullah, S.N.; Farmer, E.A.; Spargo, L.; Logan, R.; Gully, N.; Anaerobe 23, 102-108 (2013)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.15
additional information
1 mM tosyl phenylalanyl chloromethyl ketone and 0.043 mM leupeptin do not inhibit enzyme activity
Porphyromonas gingivalis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.15
cell surface
90% of enzyme activity is cell surface associated
Porphyromonas gingivalis
9986
-
3.5.3.15
cytoplasm
10% of enzyme activity is cytoplasn associated
Porphyromonas gingivalis
5737
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.15
Porphyromonas gingivalis
-
-
-
3.5.3.15
Porphyromonas gingivalis W50
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.15
agmatine + H2O
5.4% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
fibrin L-arginine + H2O
-
733075
Porphyromonas gingivalis
fibrin L-citrulline + NH3
-
-
-
?
3.5.3.15
fibrin L-arginine + H2O
-
733075
Porphyromonas gingivalis W50
fibrin L-citrulline + NH3
-
-
-
?
3.5.3.15
Gly-Gly-L-Arg + H2O
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
Gly-Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-Gly-L-Arg + H2O
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
Gly-Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-L-Arg + H2O
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-L-Arg + H2O
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
human vimentin L-arginine + H2O
-
733075
Porphyromonas gingivalis
human vimentin L-citrulline + NH3
-
-
-
?
3.5.3.15
L-Arg-Gly + H2O
75% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Cit-Gly + NH3
-
-
-
?
3.5.3.15
L-Arg-Gly-Gly + H2O
66% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Cit-Gly-Gly + NH3
-
-
-
?
3.5.3.15
L-arginine + H2O
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-citrulline + NH3
-
-
-
?
3.5.3.15
L-arginine + H2O
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
L-citrulline + NH3
-
-
-
?
3.5.3.15
L-canavanine + H2O
0.3% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
L-homoarginine + H2O
0.3% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
L-Leu-Trp-Met-Arg + H2O
45% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Leu-Trp-Met-Cit + NH3
-
-
-
?
3.5.3.15
L-Met-Arg-Phe + H2O
the substrate shows fastest rate of citrullination with 158% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Met-Cit-Phe + NH3
-
-
-
?
3.5.3.15
L-octopine + H2O
16.7% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
Nalpha-benzoyl-L-arginine ethyl ester + H2O
100% activity
733075
Porphyromonas gingivalis
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
3.5.3.15
Nalpha-benzoyl-L-arginine ethyl ester + H2O
100% activity
733075
Porphyromonas gingivalis W50
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
3.5.3.15
yeast enolase L-arginine + H2O
-
733075
Porphyromonas gingivalis
yeast enolase L-citrulline + NH3
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.15
60
-
enzyme activity decreases by 13% following exposure of cells to 60°C for 10 min
Porphyromonas gingivalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.15
7.5
8
-
Porphyromonas gingivalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.15
additional information
1 mM tosyl phenylalanyl chloromethyl ketone and 0.043 mM leupeptin do not inhibit enzyme activity
Porphyromonas gingivalis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.15
cell surface
90% of enzyme activity is cell surface associated
Porphyromonas gingivalis
9986
-
3.5.3.15
cytoplasm
10% of enzyme activity is cytoplasn associated
Porphyromonas gingivalis
5737
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.15
agmatine + H2O
5.4% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
fibrin L-arginine + H2O
-
733075
Porphyromonas gingivalis
fibrin L-citrulline + NH3
-
-
-
?
3.5.3.15
fibrin L-arginine + H2O
-
733075
Porphyromonas gingivalis W50
fibrin L-citrulline + NH3
-
-
-
?
3.5.3.15
Gly-Gly-L-Arg + H2O
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
Gly-Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-Gly-L-Arg + H2O
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
Gly-Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-L-Arg + H2O
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
Gly-L-Arg + H2O
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
Gly-L-Cit + NH3
-
-
-
?
3.5.3.15
human vimentin L-arginine + H2O
-
733075
Porphyromonas gingivalis
human vimentin L-citrulline + NH3
-
-
-
?
3.5.3.15
L-Arg-Gly + H2O
75% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Cit-Gly + NH3
-
-
-
?
3.5.3.15
L-Arg-Gly-Gly + H2O
66% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Cit-Gly-Gly + NH3
-
-
-
?
3.5.3.15
L-arginine + H2O
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-citrulline + NH3
-
-
-
?
3.5.3.15
L-arginine + H2O
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis W50
L-citrulline + NH3
-
-
-
?
3.5.3.15
L-canavanine + H2O
0.3% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
L-homoarginine + H2O
0.3% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
L-Leu-Trp-Met-Arg + H2O
45% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Leu-Trp-Met-Cit + NH3
-
-
-
?
3.5.3.15
L-Met-Arg-Phe + H2O
the substrate shows fastest rate of citrullination with 158% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
733075
Porphyromonas gingivalis
L-Met-Cit-Phe + NH3
-
-
-
?
3.5.3.15
L-octopine + H2O
16.7% activity compared to L-arginine
733075
Porphyromonas gingivalis
? + NH3
-
-
-
?
3.5.3.15
Nalpha-benzoyl-L-arginine ethyl ester + H2O
100% activity
733075
Porphyromonas gingivalis
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
3.5.3.15
Nalpha-benzoyl-L-arginine ethyl ester + H2O
100% activity
733075
Porphyromonas gingivalis W50
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
3.5.3.15
yeast enolase L-arginine + H2O
-
733075
Porphyromonas gingivalis
yeast enolase L-citrulline + NH3
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.15
60
-
enzyme activity decreases by 13% following exposure of cells to 60°C for 10 min
Porphyromonas gingivalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.15
7.5
8
-
Porphyromonas gingivalis
General Information
EC Number
General Information
Commentary
Organism
3.5.3.15
physiological function
enzyme's presence in inflamed tissue may promote autoimmune reactions by creation of altered host epitopes
Porphyromonas gingivalis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.3.15
physiological function
enzyme's presence in inflamed tissue may promote autoimmune reactions by creation of altered host epitopes
Porphyromonas gingivalis