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Literature summary extracted from

  • Homaei, A.; Barkheh, H.; Sariri, R.; Stevanato, R.
    Immobilized papain on gold nanorods as heterogeneous biocatalysts (2014), Amino Acids, 46, 1649-1657.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.2 biotechnology immobilisation of papain on gold nanorods enhances enzyme stability and efficiency, opening new opportunities for biotechnological applications Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 Ba2+ residual activity in the presence of 20 mM: 13% free papain, 38% immobilized papain Carica papaya
3.4.22.2 Ca2+ residual activity in the presence of 20 mM: 10% free papain, 48% immobilized papain Carica papaya
3.4.22.2 Cu2+ residual activity in the presence of 20 mM: 0% free papain, 20% immobilized papain Carica papaya
3.4.22.2 Mg2+ residual activity in the presence of 20 mM: 20% free papain, 57% immobilized papain Carica papaya
3.4.22.2 Mn2+ residual activity in the presence of 20 mM: 0% free papain, 18% immobilized papain Carica papaya
3.4.22.2 Ni2+ residual activity in the presence of 20 mM: 0% free papain, 16% immobilized papain Carica papaya
3.4.22.2 Zn2+ residual activity in the presence of 20 mM: 0% free papain, 16% immobilized papain Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.00061
-
 casein pH 9, 20°C, free papain Carica papaya
3.4.22.2 0.00066
-
 casein pH 9, 20°C, immobilized papain Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 casein + H2O
-
 Carica papaya ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 20
-
 assay at Carica papaya
3.4.22.2 60
-
 optimal pH free papain Carica papaya
3.4.22.2 70
-
 optimal pH immobilized papain Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.2 4
-
 residual activity after 30 storage: 0% free papain, 80% immobilized papain Carica papaya
3.4.22.2 22
-
 residual activity after 30 storage: 25% free papain, 80% immobilized papain Carica papaya
3.4.22.2 30
-
 residual activity: 70% free papain, 40% immobilized papain Carica papaya
3.4.22.2 90
-
 half life after 60 storage: 5% free papain, 30% immobilized papain Carica papaya
3.4.22.2 90
-
 residual activity: 30% free papain, 80% immobilized papain Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.0000038
-
 casein pH 9, 20°C, immobilized papain Carica papaya
3.4.22.2 0.0000046
-
 casein pH 9, 20°C, free papain Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6.9
-
 optimal pH free papain Carica papaya
3.4.22.2 9
-
 assay at Carica papaya
3.4.22.2 9
-
 optimal pH immobilized papain Carica papaya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.2 1 2 half life after 60 storage: below 5% free papain, 40% immobilized papain Carica papaya
3.4.22.2 2
-
 residual activity: 10% free papain, 40% immobilized papain Carica papaya
3.4.22.2 3
-
 half life after 60 storage: below 5% free papain, 40% immobilized papain Carica papaya
3.4.22.2 10
-
 residual activity: 20% free papain, 50% immobilized papain Carica papaya

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.2 0.0058
-
 casein pH 9, 20°C, immobilized papain Carica papaya
3.4.22.2 0.0076
-
 casein pH 9, 20°C, free papain Carica papaya