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Literature summary extracted from

  • Huang H.-K, H.H.; Chen L.-F, C.L.; Tokashiki M, T.M.; Ozawa T, O.T.; Taira T, T.T.; Ito S, I.S.
    An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii (2012), AMB Express, 2, 4.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.102 cysteic acid 1 mM, 151% of initial activity Meyerozyma guilliermondii
4.1.1.102 D-cysteine 1 mM, 150% of initial activity Meyerozyma guilliermondii
4.1.1.102 dithiothreitol 0.2-1 mM, slightly enhances decarboxylation of ferulic acid without affecteing decarboxylation of 4-coumarate Meyerozyma guilliermondii
4.1.1.102 DL-homoserine 1 mM, 160% of initial activity Meyerozyma guilliermondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.102 expression in Escherichia coli Meyerozyma guilliermondii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.102 modeling of structure based on PDB entry 2GC9. Residue Met57 is located at the entrance of the pocket and in the immediate vicinity of possible catalytic residues Arg60 and Glu82. Residue Met103 is spatially more distant from the two catalytic residues and located deeper in the active-site pocket Meyerozyma guilliermondii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.102 M57A almost complete loss of activity Meyerozyma guilliermondii
4.1.1.102 M57L mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate Meyerozyma guilliermondii
4.1.1.102 M57T almost complete loss of activity Meyerozyma guilliermondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.102 19828
-
 x * 19828, calculated Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.102 4-coumarate Meyerozyma guilliermondii
-
 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-coumarate Meyerozyma guilliermondii ATCC 9058
-
 4-vinylphenol + CO2
-
 ?
4.1.1.102 ferulate Meyerozyma guilliermondii
-
 4-vinylguaiacol + CO2
-
 ?
4.1.1.102 ferulate Meyerozyma guilliermondii ATCC 9058
-
 4-vinylguaiacol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.102 Meyerozyma guilliermondii G3XHW5
-
-
4.1.1.102 Meyerozyma guilliermondii ATCC 9058 G3XHW5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.102 20.1
-
 mutant M57L, substrate ferulate, 25°C, pH 6.0 Meyerozyma guilliermondii
4.1.1.102 378
-
 wild-type, substrate ferulate, 25°C, pH 6.0 Meyerozyma guilliermondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.102 4-coumarate
-
 Meyerozyma guilliermondii 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-coumarate 104% of the activity with ferulate Meyerozyma guilliermondii 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-coumarate
-
 Meyerozyma guilliermondii ATCC 9058 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-coumarate 104% of the activity with ferulate Meyerozyma guilliermondii ATCC 9058 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-hydroxycinnamate
-
 Meyerozyma guilliermondii 4-hydroxystyrene + CO2
-
 ?
4.1.1.102 4-hydroxycinnamate
-
 Meyerozyma guilliermondii ATCC 9058 4-hydroxystyrene + CO2
-
 ?
4.1.1.102 caffeate
-
 Meyerozyma guilliermondii 3,4-dihydroxystyrene + CO2 14% of the activity with ferulate ?
4.1.1.102 caffeate
-
 Meyerozyma guilliermondii ATCC 9058 3,4-dihydroxystyrene + CO2 14% of the activity with ferulate ?
4.1.1.102 ferulate
-
 Meyerozyma guilliermondii 4-vinylguaiacol + CO2
-
 ?
4.1.1.102 ferulate
-
 Meyerozyma guilliermondii ATCC 9058 4-vinylguaiacol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.102 ? x * 19828, calculated Meyerozyma guilliermondii

Synonyms

EC Number Synonyms Comment Organism
4.1.1.102 phenolic acid decarboxylase
-
 Meyerozyma guilliermondii

General Information

EC Number General Information Comment Organism
4.1.1.102 physiological function an endogenous amino thiol-like compound with a molecular weight greater than 1400 Da activates Meyerozyma guilliermondii