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Literature summary extracted from

  • Diao, W.; Yang, X.; Zhou, H.
    Purification, crystallization and preliminary X-ray data collection of the N-terminal domain of the 26S proteasome regulatory subunit p27 and its complex with the ATPase domain of Rpt5 from Mus musculus (2014), Acta crystallogr. Sect. F, 70, 611-615.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.5 recombinant expression of His-tagged N-terminus of p27 (residues 1-128) and the C-terminal ATPase domain of Rpt5 (residues 173-442) in a mixed cultre of tranformed Escherichia coli strain BL21(DE3) cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.1.5 purified recombinant N-terminus of p27 (residues 1-128) in complex with the C-terminal ATPase domain of Rpt5 (residues 173-442), sitting drop method, mixing of 0.001 ml of 4 mg/ml protein in 10 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, with 0.001 ml of precipitant solution containing 2.0 M NaCl, 10% PEG 6000, X-ray diffraction structure determination and analysis at 1.7 A and 4.0 A resolution, respectively Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.5 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.5 ATP + H2O + polypeptide Mus musculus
-
 ADP + phosphate + unfolded polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.5 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.5 recombinant expression of His-tagged C-terminal ATPase domain of Rpt5 (residues 173-442) from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration as protein complex with the N-terminus of p27 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.5 ATP + H2O + polypeptide
-
 Mus musculus ADP + phosphate + unfolded polypeptide
-
 ?
5.6.1.5 ATP + H2O + polypeptide C-terminal ATPase domain of Rpt5, residues 173-442 Mus musculus ADP + phosphate + unfolded polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.5 26S protease regulatory subunit 6A
-
 Mus musculus
5.6.1.5 Rpt5
-
 Mus musculus

General Information

EC Number General Information Comment Organism
5.6.1.5 metabolism the RP is composed of two subcomplexes: the base and lid. Three non-ATPase subunits, Rpn1, Rpn2 and Rpn13, and six AAA-ATPase subunits, Rpt1-6, which contain four helices at their C-termini, form the base that is attached to the CP alpha-ring, and other non-ATPase subunits form the lid. Rpt5, i.e. 26S protease regulatory subunit 6A, is one of the six ATPase subunits of the regulatory particle, RP, one of two subcomplexes of the 26S proteasome Mus musculus