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Literature summary extracted from

  • Wang, R.; Rajagopalan, K.; Sadre-Bazzaz, K.; Moreau, M.; Klessig, D.; Tong, L.
    Structure of the Arabidopsis thaliana TOP2 oligopeptidase (2014), Acta Crystallogr. Sect. F, 70, 555-559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.70 N-terminally His-tagged isozyme overexpression in Escherichia coli BL21 Rosetta (DE3) Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.70 purified enzyme TOP2 bound to 4-azido-salicylate, sitting-drop vapor-diffusion method, mixing of 40 mg/ml protein in 20 mM Tris, pH 8.5, 200 mM NaCl, 5 mM DTT, 5% v/v glycerol, with reservoir solution consisting of 220 mM ammonium acetate, 20% w/v PEG 3350, 20°C, X-ray diffraction structure determination and analysis at 3.0 A resolution Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.70 salicylic acid inhibits the peptidase activity of isozyme TOP1 and to a lesser extent isozyme TOP2; inhibits the peptidase activity of isozyme TOP1 and to a lesser extent isozyme TOP2 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.70 Zn2+ a zinc-dependent metallopeptidase Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.70 Arabidopsis thaliana Q949P2 isozyme TOP2; isozyme TOP2
-
3.4.24.70 Arabidopsis thaliana Q94AM1 isozyme TOP1; isozyme TOP1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.70 recombinant His-tagged isozyme from Escherichia coli BL21 Rosetta (DE3) by nickel affinity chromatography and gel filtration Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.4.24.70 TOP
-
 Arabidopsis thaliana
3.4.24.70 Top1
-
 Arabidopsis thaliana
3.4.24.70 Top2
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.4.24.70 evolution the TOP isozymes contain the conserved His-Glu-XX-His (HEXXH) sequence of active-site residues and belong to the M3 family of metallopeptidases Arabidopsis thaliana
3.4.24.70 physiological function the enzyme is a target for salicylic acid binding and participate in SA-mediated plant innate immunity. Both isozymes TOP1 and TOP2 seem to play a role in salicylic acid-dependent innate immunity, but through independent pathways Arabidopsis thaliana