Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, M.; Wang, L.; Zhang, H.; Dong, Y.; Gong, Y.; Zhang, L.; Wang, J.
    Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli (2014), Acta Crystallogr. Sect. F, 70, 1256-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.198 expressed in Escherichia coli BL21 (DE3) Star cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.198 in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.198 31000
-
 x * 31000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.198 S-adenosyl-L-methionine + cytidine1402 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
-
 ?
2.1.1.199 S-adenosyl-L-methionine + cytosine1402 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.198 Escherichia coli
-
-
-
2.1.1.199 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.198 Ni-NTA column chromatography, heparin column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.198 S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
-
 ?
2.1.1.199 S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.198 ? x * 31000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.198 RsmI
-
 Escherichia coli
2.1.1.199 RsmH
-
 Escherichia coli