Literature summary extracted from
Kumar, A.; Kumar, S.; Taneja, B.
The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis (2014), Acta Crystallogr. Sect. D, 70, 821-832.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.193 |
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells |
Mycobacterium tuberculosis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.1.1.193 |
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24°C |
Mycobacterium tuberculosis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.1.193 |
additional information |
the deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity |
Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.1.1.193 |
S-adenosyl-L-methionine + uracil1498 in 16S rRNA |
Mycobacterium tuberculosis |
- |
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.193 |
Mycobacterium tuberculosis |
P9WGX1 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.193 |
Ni-NTA column chromatography and Mono S column chromatography |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.193 |
S-adenosyl-L-methionine + uracil1498 in 16S rRNA |
- |
Mycobacterium tuberculosis |
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.1.193 |
dimer |
x-ray crystallography |
Mycobacterium tuberculosis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.1.193 |
RsmE |
- |
Mycobacterium tuberculosis |
2.1.1.193 |
Rv2372c |
- |
Mycobacterium tuberculosis |