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Literature summary extracted from

  • Broadley, S.G.; Gumbart, J.C.; Weber, B.W.; Marakalala, M.J.; Steenkamp, D.J.; Sewell, B.T.
    A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism (2012), Acta Crystallogr. Sect. D, 68, 1450-1459.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.103 recombinant enzyme expression Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.103 purified recombinant enzyme, from 0.1 M sodium acetate, 0.2 M ammonium sulfate, 25% PEG 4000 pH 4.6, with glycerol and the reaction product acetate in the active site, X-ray diffraction structure determination and analysis at 1.94 A resolution, modelling Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.103 Zn2+ a zinc-dependent enzyme, binding structure of the catalytic zinc, overview Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.103 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + H2O Mycobacterium tuberculosis
-
 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.103 Mycobacterium tuberculosis P9WJN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.103 recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.103 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + H2O
-
 Mycobacterium tuberculosis 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.103 MshB
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.1.103 metabolism the zinc-based deacetylase catalyses a step in the mycothiol biosynthetic pathway that involves the deacetylation of 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol via cleavage of an amide bond, to 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol and acetate Mycobacterium tuberculosis
3.5.1.103 additional information configuration strongly suggests that Asp15 acts both as a general base catalyst in the nucleophilic attack of water on the amide carbonyl C atom and in its protonated form acts as a general acid to protonate the amide N atom. Residue Tyr142 is involved in a conformational change on substrate binding and contributes to the oxyanion hole that stabilizes the tetrahedral intermediate Mycobacterium tuberculosis