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Literature summary extracted from

  • Cunha, S.; dAvo, A.F.; Mingote, A.; Lamosa, P.; da Costa, M.S.; Costa, J.
    Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica (2013), Sci. Rep., 3, 2378.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.266 expression in Escherichia coli Rhodopirellula baltica
2.4.1.270 expression in Escherichia coli Rhodopirellula baltica
3.1.3.B8 expressed in Escherichia coli Rhodopirellula baltica
3.1.3.B8 expression in Escherichia coli Rhodopirellula baltica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.266 EDTA 2.5 mM, 19% residual activity Rhodopirellula baltica
2.4.1.270 EDTA 5 mM, 47% residual activity Rhodopirellula baltica
3.1.3.B8 EDTA 2 mM, no residual activity Rhodopirellula baltica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.266 0.4
-
 3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica
2.4.1.266 0.7
-
 ADP-glucose pH 7.5, 25°C Rhodopirellula baltica
2.4.1.270 0.28
-
 GDP-mannose pH 7.5, 40°C Rhodopirellula baltica
2.4.1.270 0.41
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica
3.1.3.B8 0.22
-
 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica
3.1.3.B8 0.22
-
 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7, 25°C Rhodopirellula baltica
3.1.3.B8 6.16
-
 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7.5, 40°C Rhodopirellula baltica
3.1.3.B8 6.16
-
 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7, 40°C Rhodopirellula baltica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.266 Co2+ and Mn2+, stimulation Rhodopirellula baltica
2.4.1.266 Mg2+ maximum activtiy in presence of 1 mM Mg2+ Rhodopirellula baltica
2.4.1.266 Mn2+ and Co2+, stimulation Rhodopirellula baltica
2.4.1.270 Ca2+ at 10 mM, high stimulation Rhodopirellula baltica
2.4.1.270 Co2+ at 1 mM, high stimulation Rhodopirellula baltica
2.4.1.270 Mn2+ maximum activtiy in presence of 1 mM Mn2+ Rhodopirellula baltica
3.1.3.B8 Co2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ Rhodopirellula baltica
3.1.3.B8 Mg2+ highest activity with 10 mM Mg2+ Rhodopirellula baltica
3.1.3.B8 Mg2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+. Maximum activity in presence of 10 mM Mg2+ Rhodopirellula baltica
3.1.3.B8 Ni2+
-
 Rhodopirellula baltica
3.1.3.B8 Ni2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ Rhodopirellula baltica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.266 44990
-
 5 * 44990, calculated Rhodopirellula baltica
2.4.1.266 218600
-
 gel filtration Rhodopirellula baltica
2.4.1.270 59700
-
 2 * 59700, calculated Rhodopirellula baltica
2.4.1.270 121900
-
 gel filtration Rhodopirellula baltica
3.1.3.B8 35900
-
 x * 35900, calculated Rhodopirellula baltica
3.1.3.B8 36000
-
 SDS-PAGE Rhodopirellula baltica

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Rhodopirellula baltica Q7UXY8
-
-
2.4.1.266 Rhodopirellula baltica DSM 10527 Q7UXY8
-
-
2.4.1.270 Rhodopirellula baltica Q7URP0
-
-
2.4.1.270 Rhodopirellula baltica DSM 10527 Q7URP0
-
-
3.1.3.B8 Rhodopirellula baltica
-
-
-
3.1.3.B8 Rhodopirellula baltica Q7UIS7
-
-
3.1.3.B8 Rhodopirellula baltica DSM 10527 Q7UIS7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.266 0.03
-
 substrate GDP-glucose, pH 7.5, 25°C Rhodopirellula baltica
2.4.1.266 0.17
-
 substrate UDP-glucose, pH 7.5, 25°C Rhodopirellula baltica
2.4.1.266 1.1
-
 substrate ADP-glucose, pH 7.5, 25°C Rhodopirellula baltica
2.4.1.270 1.57
-
 substrate ADP-mannose, pH 7.5, 25°C Rhodopirellula baltica
2.4.1.270 1.72
-
 substrate UDP-mannose, pH 7.5, 25°C Rhodopirellula baltica
2.4.1.270 3.1
-
 substrate GDP-mannose, pH 7.5, 25°C Rhodopirellula baltica
3.1.3.B8 0.013
-
 pH 7.5, 25°C Rhodopirellula baltica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 additional information enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate Rhodopirellula baltica ?
-
 ?
2.4.1.266 additional information enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate Rhodopirellula baltica DSM 10527 ?
-
 ?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 ADP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica ADP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 ADP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 ADP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica ?
-
 ?
2.4.1.270 additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica DSM 10527 ?
-
 ?
2.4.1.270 UDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica UDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.270 UDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 UDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
3.1.3.B8 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica 2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
3.1.3.B8 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica DSM 10527 2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
3.1.3.B8 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
3.1.3.B8 additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica ?
-
 ?
3.1.3.B8 additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica DSM 10527 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.266 pentamer 5 * 44990, calculated Rhodopirellula baltica
2.4.1.270 dimer 2 * 59700, calculated Rhodopirellula baltica
3.1.3.B8 ? x * 35900, calculated Rhodopirellula baltica

Synonyms

EC Number Synonyms Comment Organism
2.4.1.266 GpgS
-
 Rhodopirellula baltica
2.4.1.266 RB1005
-
 Rhodopirellula baltica
2.4.1.270 MggA
-
 Rhodopirellula baltica
2.4.1.270 RB5546
-
 Rhodopirellula baltica
3.1.3.B8 MggB
-
 Rhodopirellula baltica
3.1.3.B8 MGPG phosphatase
-
 Rhodopirellula baltica
3.1.3.B8 RB12347
-
 Rhodopirellula baltica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.266 60
-
-
 Rhodopirellula baltica
2.4.1.270 40
-
-
 Rhodopirellula baltica
3.1.3.B8 40
-
-
 Rhodopirellula baltica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.270 25
-
 40% of maximum activity Rhodopirellula baltica
2.4.1.270 90
-
 18% of maximum activity Rhodopirellula baltica
3.1.3.B8 15
-
 no activity below 15°C Rhodopirellula baltica
3.1.3.B8 25
-
 28% of maximum activity Rhodopirellula baltica
3.1.3.B8 90
-
 18% of maximum activity Rhodopirellula baltica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.270 25
-
 half-life 38.5 h Rhodopirellula baltica
2.4.1.270 40
-
 half-life 52.8 min Rhodopirellula baltica
3.1.3.B8 25
-
 half-live: 9.6 hours Rhodopirellula baltica
3.1.3.B8 25
-
 half-life 9.6 h Rhodopirellula baltica
3.1.3.B8 40
-
 half-live: 5.3 hours Rhodopirellula baltica
3.1.3.B8 40
-
 half-life 5.3 h Rhodopirellula baltica
3.1.3.B8 60
-
 28% activity retained Rhodopirellula baltica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.266 7.5
-
-
 Rhodopirellula baltica
2.4.1.270 7.5
-
-
 Rhodopirellula baltica
3.1.3.B8 6
-
-
 Rhodopirellula baltica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.B8 4 9
-
 Rhodopirellula baltica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.266 Rhodopirellula baltica calculated
-
 5.4
2.4.1.270 Rhodopirellula baltica calculated
-
 5.5
3.1.3.B8 Rhodopirellula baltica calculated
-
 5.7