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Literature summary extracted from

  • Joergensen, C.M.; Madsen, S.M.; Vrang, A.; Hansen, O.C.; Johnsen, M.G.
    Recombinant expression of Laceyella sacchari thermitase in Lactococcus lactis (2013), Protein Expr. Purif., 92, 148-155.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.66 recombinant expression in Lactococcus lactis strain P170 from vector pAMJ2008 and in strain MG1363, secretion of stable thermitase in an auto-induced fermentation setup at 30°C. The enzyme accumulates in the culture supernatant during batch fermentations and is easily activated at 50°C or by prolonged dialysis Laceyella sacchari

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.66 Ca2+ required Laceyella sacchari

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.66 8500
-
 recombinant enzyme, gel filtration, thermitase interactions with the column matrix can delay elution of the protein and lead to underestimation of the molecular size Laceyella sacchari

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.66 Laceyella sacchari
-
-
-
3.4.21.66 Laceyella sacchari DSM 43353
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.66 recombinant enzyme from culture supernatant by dialysis and hydrophobic interaction chromatography Laceyella sacchari

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.66 1483
-
 purified recombinant enzyme, pH 8.0, 60°C Laceyella sacchari

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.66 azocasein + H2O
-
 Laceyella sacchari ?
-
 ?
3.4.21.66 azocasein + H2O
-
 Laceyella sacchari DSM 43353 ?
-
 ?
3.4.21.66 additional information a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity Laceyella sacchari ?
-
 ?
3.4.21.66 additional information a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity Laceyella sacchari DSM 43353 ?
-
 ?
3.4.21.66 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Laceyella sacchari N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.66 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Laceyella sacchari DSM 43353 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.66 60
-
 assay at Laceyella sacchari

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.66 50 80 activity range, recombinant enzyme Laceyella sacchari

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.66 50
-
 recombinant enzyme, stable up to Laceyella sacchari

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.66 10
-
-
 Laceyella sacchari

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.66 6 11 activity range, recombinant enzyme Laceyella sacchari

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.66 6 11 purified recombinant enzyme, 50°C, 5 h, over 60% activity Laceyella sacchari