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Literature summary extracted from

  • Wang, H.C.; Chen, Y.C.; Huang, C.T.; Hseu, R.S.
    Cloning and characterization of a thermostable and pH-stable cellobiohydrolase from Neocallimastix patriciarum J11 (2013), Protein Expr. Purif., 90, 153-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Escherichia coli Rosetta-gami B (DE3) cells Neocallimastix patriciarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 Ag+ 9% residual activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Hg2+ 4% residual activity at 1 mM, complete inhibition at 10 mM Neocallimastix patriciarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.91 Ba2+ 120% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Ca2+ 121% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Co2+ 146% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Cu2+ 127% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Fe2+ 117% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Fe3+ 138% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 Mn2+ 116% activity at 1 mM Neocallimastix patriciarum
3.2.1.91 additional information not activated by Mg2+ and Li+ Neocallimastix patriciarum
3.2.1.91 Zn2+ 115% activity at 1 mM Neocallimastix patriciarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 53950
-
 x * 53950, calculated from amino acid sequence Neocallimastix patriciarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Neocallimastix patriciarum
-
-
-
3.2.1.91 Neocallimastix patriciarum J11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 Ni-NTA agarose column chromatography and T7-tag antibody agarose column chromatography Neocallimastix patriciarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 1.1
-
 after 23.1fold purification, at pH 6.0 and 37°C for 15 min using avicel as substrate Neocallimastix patriciarum
3.2.1.91 3.3
-
 after 23.1fold purification, at pH 6.0 and 37°C for 15 min using phosphoric acid-swollen avicel as substrate Neocallimastix patriciarum
3.2.1.91 8.7
-
 crude enzyme, at pH 6.0 and 37°C for 15 min using 0.5% lichenan as substrate Neocallimastix patriciarum
3.2.1.91 108
-
 after 23.1fold purification, at pH 6.0 and 37°C for 15 min using carboxymethyl cellulose as substrate Neocallimastix patriciarum
3.2.1.91 317
-
 after 23.1fold purification, at pH 6.0 and 37°C for 15 min using lichenan as substrate Neocallimastix patriciarum
3.2.1.91 1809
-
 after 23.1fold purification, at pH 6.0 and 37°C for 15 min using barley beta-glucan as substrate Neocallimastix patriciarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Neocallimastix patriciarum 4-methylumbelliferone + cellobiose
-
 ?
3.2.1.91 avicel + H2O low specific activity Neocallimastix patriciarum cellobiose
-
 ?
3.2.1.91 avicel + H2O low specific activity Neocallimastix patriciarum J11 cellobiose
-
 ?
3.2.1.91 barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum ?
-
 ?
3.2.1.91 barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum J11 ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum J11 ?
-
 ?
3.2.1.91 lichenan + H2O high specific activity Neocallimastix patriciarum ?
-
 ?
3.2.1.91 lichenan + H2O high specific activity Neocallimastix patriciarum J11 ?
-
 ?
3.2.1.91 additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum ?
-
 ?
3.2.1.91 additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum J11 ?
-
 ?
3.2.1.91 phosphoric acid-swollen Avicel + H2O low specific activity Neocallimastix patriciarum cellobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 53950, calculated from amino acid sequence Neocallimastix patriciarum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CelA
-
 Neocallimastix patriciarum
3.2.1.91 cellobiohydrolase
-
 Neocallimastix patriciarum
3.2.1.91 exo-1,4-beta-D-glucan cellobiohydrolase
-
 Neocallimastix patriciarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 50
-
-
 Neocallimastix patriciarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.91 30 55 about 60% activity at 30°C, about 55% activity at 35°C, about 80% activity at 40°C, about 75% activity at 45°C, 100% activity at 50°C, about 40% activity at 55°C, and almost no activity at 60°C Neocallimastix patriciarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 60 70 the enzyme maintains approximately 50% of the maximum activity in response to the treatment at 60-70°C for 1 h Neocallimastix patriciarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 6
-
-
 Neocallimastix patriciarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.91 5 7.5 more than 50% activity between pH 5.0 and 7.5 Neocallimastix patriciarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.91 5.2 11.3 the enzyme is stable over a wide range of pH 5.2-11.3 after 1 h incubation (more than 70% activity remaining) Neocallimastix patriciarum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.91 Neocallimastix patriciarum calculated from amino acid sequence
-
 4.7