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Literature summary extracted from

  • Novinec, M.; Pavsic, M.; Lenarcic, B.
    A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coli (2012), Protein Expr. Purif., 82, 1-5.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.43 additional information the recombinant proenzyme is autocatalytically activated in vitro by incubation at pH 4.0 and 30°C Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.43 recombinant procathepsin V is expressed in soluble form in the cytoplasm of Escherichia coli Rosetta-gami B (DE3) pLysS cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.43 E-64 irreversible Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.43 0.0024
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
3.4.22.43 0.0044
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.43 37000
-
 x * 37000, recombinant procathepsin V, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.43 plasminogen + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.43 Homo sapiens O60911
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.43 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.43 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.43 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.43 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.43 plasminogen + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.43 ? x * 37000, recombinant procathepsin V, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.43 19
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
3.4.22.43 156
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.43 7900
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
3.4.22.43 35000
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens