EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.20 | expression in Escherichia coli | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.11.20 | additional information | synthesis and cloning of an optimized gene, with a codon adaptation index and an average G/C content higher than the native gene. Optimal expression is achieved by induction with 1 mM IPTG at 37°C for 18 h. This allows obtaining 100 mg of recombinant enzyme per liter of culture medium. The protein shows only limited signs of proteolytic degradation, and purification in a single step by immobilized metal ion affinity chromatography increases purity 27fold | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.20 | 1,10-phenanthroline | 1 mM, 80% inhibition | Plasmodium falciparum | |
3.4.11.20 | Zn2+ | 0.1 mM, 80% inhibition | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.20 | 0.248 | - |
L-Lys 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 0.27 | - |
L-Arg 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 0.304 | - |
L-Ala 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.20 | 106130 | - |
x * 106130, calculated for recombinant protein including His-tag | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.20 | Plasmodium falciparum | Q8IEK1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.20 | L-Ala 4-nitroanilide + H2O | - |
Plasmodium falciparum | L-Ala + 4-nitroaniline | - |
? | |
3.4.11.20 | L-Arg 4-nitroanilide + H2O | - |
Plasmodium falciparum | L-Arg + 4-nitroaniline | - |
? | |
3.4.11.20 | L-Leu 4-nitroanilide + H2O | - |
Plasmodium falciparum | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.20 | L-Lys 4-nitroanilide + H2O | - |
Plasmodium falciparum | L-Lys + 4-nitroaniline | - |
? | |
3.4.11.20 | additional information | no substrates: Ile-4-nitroanilide, Val-4-nitroanilide, Gly-4-nitroanilide, Pro-4-nitroanilide | Plasmodium falciparum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.20 | ? | x * 106130, calculated for recombinant protein including His-tag | Plasmodium falciparum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.20 | 1.15 | - |
L-Ala 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 1.55 | - |
L-Lys 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 1.58 | - |
L-Arg 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 1.65 | - |
L-Leu 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.20 | 7.2 | 7.4 | - |
Plasmodium falciparum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.20 | 4.24 | - |
L-Ala 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 5.42 | - |
L-Leu 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 5.84 | - |
L-Arg 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum | |
3.4.11.20 | 6.24 | - |
L-Lys 4-nitroanilide | pH 7.2, 37°C | Plasmodium falciparum |