EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.65 | synthesis | the enzyme is uzilized for production of beta2-6 fructose oligosaccharides (levan-type FOS) through a sequential reactionwith levan produced from sucrose by bacterial levansucrases, method development, overview | Bacillus licheniformis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.65 | DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli | Bacillus licheniformis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.65 | additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus licheniformis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.65 | 56400 | - |
x * 56400, SDS-PAGE | Bacillus licheniformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.65 | levan + H2O | Bacillus licheniformis | - |
? | - |
? | |
3.2.1.65 | levan + H2O | Bacillus licheniformis IBt1 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.65 | Bacillus licheniformis | W8GV60 | - |
- |
3.2.1.65 | Bacillus licheniformis IBt1 | W8GV60 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.65 | 1.8 | - |
purified recombinant enzyme, pH 6.0, 35°C | Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.65 | levan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.65 | levan + H2O | - |
Bacillus licheniformis IBt1 | ? | - |
? | |
3.2.1.65 | levan + H2O | almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase | Bacillus licheniformis | O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ? | major product | ? | |
3.2.1.65 | levan + H2O | almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase | Bacillus licheniformis IBt1 | O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ? | major product | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.65 | ? | x * 56400, SDS-PAGE | Bacillus licheniformis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.65 | endolevanase | - |
Bacillus licheniformis |
3.2.1.65 | LevB1 | - |
Bacillus licheniformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.65 | 35 | - |
- |
Bacillus licheniformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.65 | 30 | 35 | purified recombinant enzyme, 24 h, completely stable at | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.65 | 6 | - |
- |
Bacillus licheniformis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.65 | evolution | the enzyme belongs to the glycosyl hydrolase family 32, GH32 | Bacillus licheniformis |