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Literature summary extracted from

  • Porras-Dominguez, J.; Avila-Fernandez, A.; Rodriguez-Alegri­a, M.; Miranda-Molina, A.; Escalante, A.; Gonzalez-Cervantes, R.; Olvera, C.; Lopez Mungui­a, A.
    Levan-type FOS production using a Bacillus licheniformis endolevanase (2014), Process Biochem., 49, 783-790.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.65 synthesis the enzyme is uzilized for production of beta2-6 fructose oligosaccharides (levan-type FOS) through a sequential reactionwith levan produced from sucrose by bacterial levansucrases, method development, overview Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.65 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.65 additional information
-
 additional information Michaelis-Menten kinetics Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 56400
-
 x * 56400, SDS-PAGE Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.65 levan + H2O Bacillus licheniformis
-
 ?
-
 ?
3.2.1.65 levan + H2O Bacillus licheniformis IBt1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Bacillus licheniformis W8GV60
-
-
3.2.1.65 Bacillus licheniformis IBt1 W8GV60
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 1.8
-
 purified recombinant enzyme, pH 6.0, 35°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.65 levan + H2O
-
 Bacillus licheniformis IBt1 ?
-
 ?
3.2.1.65 levan + H2O almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase Bacillus licheniformis O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ? major product ?
3.2.1.65 levan + H2O almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase Bacillus licheniformis IBt1 O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ? major product ?

Subunits

EC Number Subunits Comment Organism
3.2.1.65 ? x * 56400, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.65 endolevanase
-
 Bacillus licheniformis
3.2.1.65 LevB1
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 35
-
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.65 30 35 purified recombinant enzyme, 24 h, completely stable at Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 6
-
-
 Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.2.1.65 evolution the enzyme belongs to the glycosyl hydrolase family 32, GH32 Bacillus licheniformis