EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.130 | expressed in Escherichia coli BL21(DE3) cells | Bacteroides xylanisolvens |
3.2.1.130 | expressed in Escherichia coli BL21(DE3) cells | Bacteroides thetaiotaomicron |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.130 | single wavelength anomalous dispersion technique | Bacteroides xylanisolvens |
3.2.1.130 | single wavelength anomalous dispersion technique | Bacteroides thetaiotaomicron |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.130 | E154A | the mutant shows near zero activity | Bacteroides thetaiotaomicron |
3.2.1.130 | E329A | the mutant shows near zero activity | Bacteroides thetaiotaomicron |
3.2.1.130 | E332A | the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme | Bacteroides thetaiotaomicron |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.130 | (3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine | - |
Bacteroides thetaiotaomicron | |
3.2.1.130 | (3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine | - |
Bacteroides xylanisolvens | |
3.2.1.130 | alpha-glucopyranosyl-(1->3)-deoxymannojirimycin | - |
Bacteroides thetaiotaomicron | |
3.2.1.130 | alpha-glucopyranosyl-(1->3)-deoxymannojirimycin | - |
Bacteroides xylanisolvens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.130 | 0.48 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron | |
3.2.1.130 | 0.7 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides xylanisolvens | |
3.2.1.130 | 2.6 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | mutant enzyme E332A, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.130 | GlcMan7GlcNAc2 + H2O | Bacteroides thetaiotaomicron | - |
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | Bacteroides xylanisolvens | - |
Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | Bacteroides thetaiotaomicron DSM 2079 | - |
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | Bacteroides xylanisolvens XB1A | - |
Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | Bacteroides xylanisolvens | - |
? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | Bacteroides thetaiotaomicron | - |
? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | Bacteroides thetaiotaomicron DSM 2079 | - |
? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | Bacteroides xylanisolvens XB1A | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.130 | Bacteroides thetaiotaomicron | Q8A109 | - |
- |
3.2.1.130 | Bacteroides thetaiotaomicron DSM 2079 | Q8A109 | - |
- |
3.2.1.130 | Bacteroides xylanisolvens | D6D1V7 | - |
- |
3.2.1.130 | Bacteroides xylanisolvens XB1A | D6D1V7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.130 | NiSO4-charged HiTrap column chromatography and S75 gel filtration | Bacteroides xylanisolvens |
3.2.1.130 | NiSO4-charged HiTrap column chromatography and S75 gel filtration | Bacteroides thetaiotaomicron |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.130 | alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O | - |
Bacteroides xylanisolvens | ? | - |
? | |
3.2.1.130 | alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
3.2.1.130 | alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O | - |
Bacteroides thetaiotaomicron DSM 2079 | ? | - |
? | |
3.2.1.130 | alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O | - |
Bacteroides xylanisolvens XB1A | ? | - |
? | |
3.2.1.130 | Glc(1->3)Man9GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron | Man8GlcNAc + glucosyl alpha(1->3)-mannose | - |
? | |
3.2.1.130 | Glc(1->3)Man9GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron DSM 2079 | Man8GlcNAc + glucosyl alpha(1->3)-mannose | - |
? | |
3.2.1.130 | Glc(1->3)Man9GlcNAc2 + H2O | - |
Bacteroides xylanisolvens | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
3.2.1.130 | Glc(1->3)Man9GlcNAc2 + H2O | - |
Bacteroides xylanisolvens XB1A | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron | Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron DSM 2079 | Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | - |
Bacteroides xylanisolvens | Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan7GlcNAc2 + H2O | - |
Bacteroides xylanisolvens XB1A | Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | - |
Bacteroides xylanisolvens | ? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | - |
Bacteroides thetaiotaomicron DSM 2079 | ? | - |
? | |
3.2.1.130 | GlcMan9GlcNAc2 + H2O | - |
Bacteroides xylanisolvens XB1A | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.130 | endo-alpha-mannosidase | - |
Bacteroides xylanisolvens |
3.2.1.130 | endo-alpha-mannosidase | - |
Bacteroides thetaiotaomicron |
3.2.1.130 | GH99 | - |
Bacteroides xylanisolvens |
3.2.1.130 | GH99 | - |
Bacteroides thetaiotaomicron |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.130 | 0.58 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | mutant enzyme E332A, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron | |
3.2.1.130 | 4.77 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron | |
3.2.1.130 | 6.34 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides xylanisolvens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.130 | 0.2 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | mutant enzyme E332A, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron | |
3.2.1.130 | 9.1 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides xylanisolvens | |
3.2.1.130 | 9.9 | - |
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride | wild type enzyme, at pH 8.0 and 37°C | Bacteroides thetaiotaomicron |