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Literature summary extracted from

  • Thompson, A.J.; Williams, R.J.; Hakki, Z.; Alonzi, D.S.; Wennekes, T.; Gloster, T.M.; Songsrirote, K.; Thomas-Oates, J.E.; Wrodnigg, T.M.; Spreitz, J.; Stuetz, A.E.; Butters, T.D.; Williams, S.J.; Davies, G.J.
    Structural and mechanistic insight into N-glycan processing by endo-alpha-mannosidase (2012), Proc. Natl. Acad. Sci. USA, 109, 781-786.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.130 expressed in Escherichia coli BL21(DE3) cells Bacteroides xylanisolvens
3.2.1.130 expressed in Escherichia coli BL21(DE3) cells Bacteroides thetaiotaomicron

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.130 single wavelength anomalous dispersion technique Bacteroides xylanisolvens
3.2.1.130 single wavelength anomalous dispersion technique Bacteroides thetaiotaomicron

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.130 E154A the mutant shows near zero activity Bacteroides thetaiotaomicron
3.2.1.130 E329A the mutant shows near zero activity Bacteroides thetaiotaomicron
3.2.1.130 E332A the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.130 (3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine
-
Bacteroides thetaiotaomicron
3.2.1.130 (3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine
-
Bacteroides xylanisolvens
3.2.1.130 alpha-glucopyranosyl-(1->3)-deoxymannojirimycin
-
Bacteroides thetaiotaomicron
3.2.1.130 alpha-glucopyranosyl-(1->3)-deoxymannojirimycin
-
Bacteroides xylanisolvens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.130 0.48
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.130 0.7
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens
3.2.1.130 2.6
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.130 GlcMan7GlcNAc2 + H2O Bacteroides thetaiotaomicron
-
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O Bacteroides xylanisolvens
-
Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O Bacteroides thetaiotaomicron DSM 2079
-
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O Bacteroides xylanisolvens XB1A
-
Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O Bacteroides xylanisolvens
-
?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O Bacteroides thetaiotaomicron
-
?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O Bacteroides thetaiotaomicron DSM 2079
-
?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O Bacteroides xylanisolvens XB1A
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.130 Bacteroides thetaiotaomicron Q8A109
-
-
3.2.1.130 Bacteroides thetaiotaomicron DSM 2079 Q8A109
-
-
3.2.1.130 Bacteroides xylanisolvens D6D1V7
-
-
3.2.1.130 Bacteroides xylanisolvens XB1A D6D1V7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.130 NiSO4-charged HiTrap column chromatography and S75 gel filtration Bacteroides xylanisolvens
3.2.1.130 NiSO4-charged HiTrap column chromatography and S75 gel filtration Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.130 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
Bacteroides xylanisolvens ?
-
?
3.2.1.130 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
Bacteroides thetaiotaomicron ?
-
?
3.2.1.130 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
Bacteroides thetaiotaomicron DSM 2079 ?
-
?
3.2.1.130 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
Bacteroides xylanisolvens XB1A ?
-
?
3.2.1.130 Glc(1->3)Man9GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron Man8GlcNAc + glucosyl alpha(1->3)-mannose
-
?
3.2.1.130 Glc(1->3)Man9GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron DSM 2079 Man8GlcNAc + glucosyl alpha(1->3)-mannose
-
?
3.2.1.130 Glc(1->3)Man9GlcNAc2 + H2O
-
Bacteroides xylanisolvens Man8GlcNAc + glucosyl alpha1,3-mannose
-
?
3.2.1.130 Glc(1->3)Man9GlcNAc2 + H2O
-
Bacteroides xylanisolvens XB1A Man8GlcNAc + glucosyl alpha1,3-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron DSM 2079 Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O
-
Bacteroides xylanisolvens Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
?
3.2.1.130 GlcMan7GlcNAc2 + H2O
-
Bacteroides xylanisolvens XB1A Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O
-
Bacteroides xylanisolvens ?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron ?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O
-
Bacteroides thetaiotaomicron DSM 2079 ?
-
?
3.2.1.130 GlcMan9GlcNAc2 + H2O
-
Bacteroides xylanisolvens XB1A ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.130 endo-alpha-mannosidase
-
Bacteroides xylanisolvens
3.2.1.130 endo-alpha-mannosidase
-
Bacteroides thetaiotaomicron
3.2.1.130 GH99
-
Bacteroides xylanisolvens
3.2.1.130 GH99
-
Bacteroides thetaiotaomicron

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.130 0.58
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.130 4.77
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.130 6.34
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.130 0.2
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.130 9.1
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens
3.2.1.130 9.9
-
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron