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Literature summary extracted from

  • Jalak, J.; Vaeljamaee, P.
    Multi-mode binding of cellobiohydrolase Cel7A from Trichoderma reesei to cellulose (2014), PLoS ONE, 9, e108181.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 additional information
-
 additional information bacterial cellulose binding at low nanomolar free enzyme concentrations is exclusively active site-mediated and is consistent with Langmuir's one binding site model, strongest binding observed with non-complexed cellulases, productive binding of te enzyme to cellulose chain ends on the hydrophobic face of bacterial cellulose microfibril. With increasing free TrCel7A concentrations the isotherm gradually deviates from the Langmuir's one binding site model caused by the increasing contribution of lower affinity binding modes that included both active site-mediated binding and non-productive binding with active site free from cellulose chain. The binding of enzyme to bacterial cellulose is only partially reversible. Measurement of binding kinetics and modelling, overview Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei
-
-
-
3.2.1.176 Trichoderma reesei QM 9414
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl beta-D-lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferol + D-lactose
-
 ?
3.2.1.176 4-methylumbelliferyl beta-D-lactoside + H2O
-
 Trichoderma reesei QM 9414 4-methylumbelliferol + D-lactose
-
 ?
3.2.1.176 4-nitrophenyl beta-D-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + D-lactose
-
 ?
3.2.1.176 4-nitrophenyl beta-D-lactoside + H2O
-
 Trichoderma reesei QM 9414 4-nitrophenol + D-lactose
-
 ?
3.2.1.176 bacterial cellulose + H2O binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei ?
-
 ?
3.2.1.176 bacterial cellulose + H2O binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei QM 9414 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase
-
 Trichoderma reesei

General Information

EC Number General Information Comment Organism
3.2.1.176 additional information bacterial cellulose binding at low nanomolar free enzyme concentrations is exclusively active site-mediated and is consistent with Langmuir's one binding site model, strongest binding observed with non-complexed cellulases, productive binding of te enzyme to cellulose chain ends on the hydrophobic face of bacterial cellulose microfibril. With increasing free TrCel7A concentrations the isotherm gradually deviates from the Langmuir’s one binding site model caused by the increasing contribution of lower affinity binding modes that includes both active site-mediated binding and non-productive binding with active site free from cellulose chain. The binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei