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Literature summary extracted from

  • Ahooghalandari, P.; Hanke, N.; Thorpe, M.; Witte, A.; Messinger, J.; Hellman, L.
    Mutations in Arg143 and Lys192 of the human mast cell chymase markedly affect the activity of five potent human chymase inhibitors (2013), PLoS ONE, 8, e65988.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.39 mutant enzymes asre expressed in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.39 K192M the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens
3.4.21.39 R143Q the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens
3.4.21.39 R143Q/K192M the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.39 1-[(5-fluoro-1-benzothiophen-3-yl)methyl]-3-{[(methoxycarbonyl)(methyl)amino]methyl}-1H-indole-2-carboxylate
-
 Homo sapiens
3.4.21.39 2-{1-[(1,4-dimethyl-1H-indol-3-yl)methyl]-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl}hexanoic acid
-
 Homo sapiens
3.4.21.39 4-({1-[(4-methyl-1-benzothiophen-3-yl)methyl]-1H-benzimidazol-2-yl}sulfanyl)butanoic acid
-
 Homo sapiens
3.4.21.39 TY51184
-
 Homo sapiens
3.4.21.39 [1-(5-chloro-1-methyl-1H-indol-3-yl)-2-(naphthalen-2-ylamino)-2-oxoethyl]phosphonate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.39 26000
-
 x * 26000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.39 angiotensin I + H2O Homo sapiens
-
 angiotensin II + His-Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.39 Homo sapiens P23946
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.39 Ni-NTA agarose beads chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.39 angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
3.4.21.39 succinyl-L-Leu-L-Leu-L-Val-L-Tyr-4-nitroanilide + H2O
-
 Homo sapiens succinyl-L-Leu-L-Leu-L-Val-L-Tyr + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.39 ? x * 26000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.39 mast cell chymase
-
 Homo sapiens