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Literature summary extracted from
- Functional analyses of complement convertases using C3 and C5-depleted sera (2012), PLoS ONE, 7, e47245.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.43 | factor B | the classical C5 convertase requires factor D, factor B, and properdin for activation and complex assembly | Homo sapiens | |
| 3.4.21.43 | factor D | the classical C5 convertase requires factor D, factor B, and properdin for activation and complex assembly | Homo sapiens | |
| 3.4.21.43 | properdin | the classical C5 convertase requires factor D, factor B, and properdin for activation and complex assembly | Homo sapiens | |
| 3.4.21.47 | factor B | the classical C5 convertase requires factor D and factor B for activation and complex assembly | Homo sapiens | |
| 3.4.21.47 | factor D | the classical C5 convertase requires factor D and factor B for activation and complex assembly | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.43 | additional information | addition of guinea pig serum in 40 mM EDTA initiates lysis of existing convertase complexes and excludes the possibility of de novo convertase formation | Homo sapiens | |
| 3.4.21.43 | mouse anti human factor B antibody | - |
Homo sapiens | |
| 3.4.21.43 | thioredoxin 1 | Trx-1, but not an active site mutated form, inhibits both C3 and C5 classical convertase formation, mechanism, overview. Trx-1 is capable of inhibiting all classical and alternative convertases but its effect is more pronounced in inhibition of alternative ones | Homo sapiens |  |
| 3.4.21.47 | additional information | addition of guinea pig serum in 40 mM EDTA initiates lysis of existing convertase complexes and excludes the possibility of de novo convertase formation | Homo sapiens | |
| 3.4.21.47 | thioredoxin 1 | Trx-1, causes significant inhibition of alternative convertases, mechanism, overview. Trx-1 is capable of inhibiting all classical and alternative convertases but its effect is more pronounced in inhibition of alternative ones | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.43 | cell surface | - |
Homo sapiens | 9986 | - |
| 3.4.21.47 | cell surface | - |
Homo sapiens | 9986 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.43 | Ca2+ | dependent on | Homo sapiens | |
| 3.4.21.43 | Mg2+ | required | Homo sapiens | |
| 3.4.21.47 | Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.43 | C3 zymogen + H2O | Homo sapiens | activation | C3b + C3a | - |
? | |
| 3.4.21.43 | C5 zymogen + H2O | Homo sapiens | activation | C5b + C5a | - |
? | |
| 3.4.21.47 | complement component C3 zymogen + H2O | Homo sapiens | activation | complement component C3b + complement component C3a | - |
? | |
| 3.4.21.47 | complement component C5 zymogen + H2O | Homo sapiens | activation | complement component C5b + complement component C5a | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.43 | Homo sapiens | - |
- |
- |
| 3.4.21.47 | Homo sapiens | P00751 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.43 | erythrocyte | - |
Homo sapiens | - |
| 3.4.21.47 | erythrocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.43 | C3 zymogen + H2O | - |
Homo sapiens | C3b + C3a | - |
? | |
| 3.4.21.43 | C3 zymogen + H2O | activation | Homo sapiens | C3b + C3a | - |
? | |
| 3.4.21.43 | C5 zymogen + H2O | - |
Homo sapiens | C5b + C5a | - |
? | |
| 3.4.21.43 | C5 zymogen + H2O | activation | Homo sapiens | C5b + C5a | - |
? | |
| 3.4.21.47 | complement component C3 zymogen + H2O | - |
Homo sapiens | complement component C3b + complement component C3a | - |
? | |
| 3.4.21.47 | complement component C3 zymogen + H2O | activation | Homo sapiens | complement component C3b + complement component C3a | - |
? | |
| 3.4.21.47 | complement component C5 zymogen + H2O | - |
Homo sapiens | complement component C5b + complement component C5a | - |
? | |
| 3.4.21.47 | complement component C5 zymogen + H2O | activation | Homo sapiens | complement component C5b + complement component C5a | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.43 | C3 convertase | - |
Homo sapiens |
| 3.4.21.43 | C4b2a | - |
Homo sapiens |
| 3.4.21.43 | C4b2aC3b | - |
Homo sapiens |
| 3.4.21.43 | C5 convertase | - |
Homo sapiens |
| 3.4.21.43 | classical C3 convertase | - |
Homo sapiens |
| 3.4.21.43 | classical C5 convertase | - |
Homo sapiens |
| 3.4.21.47 | alternative C3 convertase | - |
Homo sapiens |
| 3.4.21.47 | alternative C5 convertase | - |
Homo sapiens |
| 3.4.21.47 | C3 convertase | - |
Homo sapiens |
| 3.4.21.47 | C3bBb | - |
Homo sapiens |
| 3.4.21.47 | C3bBbC3b | - |
Homo sapiens |
| 3.4.21.47 | C5 convertase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.43 | 37 | - |
assay at | Homo sapiens |
| 3.4.21.47 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.43 | 7.3 | - |
assay at | Homo sapiens |
| 3.4.21.47 | 7.3 | - |
assay at | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.21.43 | metabolism | all pathways converge at the level of the C3 molecule, where downstream events can be amplified by a mechanism of positive feedback supported by complement convertases: the classical/lectin pathway C3 convertase (C4b2a) or the alternative pathway C3 convertase (C3bBb). These convertases further cleave C3 to C3b and C3a, of which C3b binds to nearby surfaces, providing a convertase assembly platform, or to pre-assembled C3 convertases, switching them to C5 convertases (C4b2aC3b or C3bBbC3b, respectively). The C5 convertase cleaves C5 molecules to C5a and C5b and the latter initiates formation of the membrane attack complex (MAC, C5b678polyC9) and its insertion into a target membrane. Enzyme regulation, overview | Homo sapiens |
| 3.4.21.43 | additional information | the classical C5 convertase requires factor D, factor B, and properdin for activation and complex assembly | Homo sapiens |
| 3.4.21.47 | metabolism | all pathways converge at the level of the C3 molecule, where downstream events can be amplified by a mechanism of positive feedback supported by complement convertases: the classical/lectin pathway C3 convertase (C4b2a) or the alternative pathway C3 convertase (C3bBb). These convertases further cleave C3 to C3b and C3a, of which C3b binds to nearby surfaces, providing a convertase assembly platform, or to pre-assembled C3 convertases, switching them to C5 convertases (C4b2aC3b or C3bBbC3b, respectively). The C5 convertase cleaves C5 molecules to C5a and C5b and the latter initiates formation of the membrane attack complex (MAC, C5b678polyC9) and its insertion into a target membrane. Enzyme regulation, overview | Homo sapiens |
| 3.4.21.47 | additional information | the classical C5 convertase requires factor D and factor B for activation and complex assembly | Homo sapiens |
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